In vitro propagation of Pyrus pyrifolia

A micropropagation method for an adult seedling tree of Pyrus pyrifolia Burm. f. is described. Shoot cultures were established and multiplied on media containing benzylamino purine and naphthaleneacetic acid, rooted on media containing naphthaleneacetic acid and phloroglucinol, and transplanted to potting mix. Addition of phloroglucinol to the rooting medium enhanced both the percentage of shoots forming roots and the survival of plantlets after transplantation. However, about 30% of the plants raised in vitro were lost owing to a shoot-collapse which was not prevented by the application of fungicides.Establishment and multiplication of shoot cultures was also achieved with 5 named cultivars of P. pyrifolia, but rooting of the shoots of these cultivars has not been satisfactory.