| 正交试验法测定锥栗总黄酮含量技术研究 |
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| 引用本文： | 郑诚乐,许伟东,潘东明,吴宪志,邱伟琴,郭亚市.正交试验法测定锥栗总黄酮含量技术研究[J].中国农学通报,2009,25(1):128-131. |
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| 作者姓名： | 郑诚乐 许伟东 潘东明 吴宪志 邱伟琴 郭亚市 |
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| 作者单位： | 福建农林大学园艺学院,福州,350002 |
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| 基金项目： | 福建省教育厅科研项目，福建省教育厅科研项目 |
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| 摘 要： | 摘要：以芦丁为对照品，采用正交试验优选法，建立锥栗中总黄酮含量的测定技术。样品采用乙醇浸提法提取，分光光度法检测，检测波长为209nm。结果表明：加样回收率为94.3％，RSD为0.61％( =6)。该试验方法简单，操作方便，可用于锥栗黄酮含量的测定。
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| 关 键 词： | 正交设计法 锥栗 总黄酮 |
| 收稿时间： | 2008-08-04 |
| 修稿时间： | 2008-11-05 |
Content determination of total flavonoids in fruit of Castanea henryi by orthogonal design |
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| Zheng Chengle,Xu Weidong,Pan Dongming,Wu Xianzhi,Qiu Weiqin,Guo Yashi.Content determination of total flavonoids in fruit of Castanea henryi by orthogonal design[J].Chinese Agricultural Science Bulletin,2009,25(1):128-131. |
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| Authors: | Zheng Chengle Xu Weidong Pan Dongming Wu Xianzhi Qiu Weiqin Guo Yashi |
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| Institution: | (College of Horticulture, FA FU, Fuzhou 350002) |
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| Abstract: | Abstract： The content of total flavonoids in fruit of Castanea henryi was distilled with ethanol and determined at 509nm by ultraviolet spectrophotometry with rutin as a standard contro1 by orthogonal design. The results show that the average recovery of total flavonoids was 94.3％ with the relative standard deviation 0.61％( =6). The method is simple，rapid and accurate. It can be used for determining the content of total flavonoids in fruit of Castanea henryi. |
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| Keywords: | orthogonal design Castanea henryi flavonoids |
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