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| 猪带绦虫TSOL18特异性单克隆抗体的制备及其体外杀虫作用的研究 | |
| 引用本文： | 郭爱疆,吴润,贾万忠,刘斌,闫鸿斌,骆学农,才学鹏.猪带绦虫TSOL18特异性单克隆抗体的制备及其体外杀虫作用的研究[J].畜牧兽医学报,2010,41(2). |
| 作者姓名： | 郭爱疆 吴润 贾万忠 刘斌 闫鸿斌 骆学农 才学鹏 |
| 作者单位： | 1. 甘肃农业大学动物医学院,兰州,730070;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部兽医公共卫生重点实验室,甘肃省动物寄生虫病重点实验室,兰州,730046 2. 甘肃农业大学动物医学院,兰州,730070 3. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部兽医公共卫生重点实验室,甘肃省动物寄生虫病重点实验室,兰州,730046 |
| 基金项目： | 国家"863"计划项目，国家科技支撑计划项目，家畜疫病病原生物学国家重点实验室项目，基本科研业务费专项资金项目 |
| 摘 要： | 本研究目的在于制备抗猪带绦虫TSOL18单克隆抗体,并分析单抗对六钩蚴的杀伤作用。采用SephadexG-100纯化在毕赤酵母中表达的猪带绦虫六钩蚴TSOL18蛋白;纯化的蛋白免疫BALB/c小鼠,运用杂交瘤技术建立能分泌抗TSOL18单克隆抗体的细胞株;通过ELISA叠加试验进行mAb的抗原表位分析;采用间接ELISA法测定TSOL18单克隆抗体特异性及腹水效价;采用抗TSOL18单克隆抗体对激活的猪带绦虫六钩蚴进行体外六钩蚴杀伤试验,观察单抗对猪带绦虫六钩蚴活力的影响。结果成功获得12株稳定分泌抗TSOL18单克隆抗体的杂交瘤细胞株,识别2个不同抗原表位,不同抗原表位的2株单克隆抗体腹水效价分别为1×102、1×106。抗体体外六钩蚴杀伤试验结果证明,在有补体的情况下,多抗和单抗作用6 d后,六钩蚴结构模糊,边缘不清晰。表明单抗对六钩蚴有一定的杀伤作用。 |
| 关 键 词： | 猪带绦虫六钩蚴 单抗 体外杀六钩蚴作用 |
Preparation of Anti-TSOL18 Monoclonal Antibodies and Their Effect on Taenia solium Oncospheres |
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| GUO Ai-jiang,WU Run,JIA Wan-zhong,LIU Bin,YAN Hong-bin,LUO Xue-nong,CAI Xue-peng.Preparation of Anti-TSOL18 Monoclonal Antibodies and Their Effect on Taenia solium Oncospheres[J].Acta Veterinaria et Zootechnica Sinica,2010,41(2). | |
| Authors: | GUO Ai-jiang WU Run JIA Wan-zhong LIU Bin YAN Hong-bin LUO Xue-nong CAI Xue-peng |
| Institution: | GUO Ai-jiang1,2,WU Run1,JIA Wan-zhong2,LIU Bin2,YAN Hong-bin2,LUO Xue-nong2,CAI Xue-peng2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of VeterinaryPublic Health of Ministry of Agriculture/State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
| Abstract: | MAbs against Taenia solium TSOL18 were prepared and their in vitro effect on oncospheres was analyzed.TSOL18 expressed in Pichia pastoris was purified with Sephadex G-100;mAbs were prepared by hybridoma technique using purified TSOL18 as an immunogen;Antigen epitopes were tested by ELISA adding test;Specificity and titer of the mAbs was determined using indirect ELISA;The effect of the mAbs on oncospheres was tested by in vitro oncospheres-killing test.Twelve hybridoma cell lines were obtained stably secret... |
| Keywords: | TSOL18 Taenia solium oncosphere TSOL18 mAb in vitro oncosphere-killing assay |
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