Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm |
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Authors: | Santiago Moreno Juan Pedro Martín Jesús María Ortiz |
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Institution: | (1) Dpto. Biología Vegetal, ETSI Agrónomos (U.P.M.), Ciudad Universitaria, 28040 Madrid, Spain |
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Abstract: | ISSR-PCR was carried out on 12 accessions of the grapevine (Vitis vinifera L.) variety 'Garnacha' and one accession of a related
variety. The analysis had two objectives: to determine if ISSR-PCR could detect polymorphisms within a variety unable to be
revealed previously with RAPDs, and to quantify the intralaboratory reproducibility of the assay. Two electrophoretic systems
and staining procedures were assessed. No intravarietal differences could be detected. However, the ISSR approach revealed
highly reproducible polymorphisms among the two varieties tested. The reproducibility of the banding patterns ranged from
86 to 94 per cent, being maximum when polyacrylamide gel electrophoresis and silver staining procedures were followed and
weak bands discarded.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | clonal characterization reproducibility simple sequence repeats Vitis vinifera |
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