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| 抗微管除草剂对玉米单倍体加倍效果研究 | |
| 引用本文： | 慈佳宾,李继竹,刘振库,尹日成,姜龙,郑雷雷,梁晓斐,杨伟光. 抗微管除草剂对玉米单倍体加倍效果研究[J]. 玉米科学, 2012, 20(5): 10-14 |
| 作者姓名： | 慈佳宾 李继竹 刘振库 尹日成 姜龙 郑雷雷 梁晓斐 杨伟光 |
| 作者单位： | 1. 吉林农业大学农学院,长春,130118 2. 长春市种子管理站,长春,130033 3. 延边州种子管理站,吉林延吉,133001 4. 吉林省农业科学院,长春,130033 |
| 基金项目： | 科技部农业科技成果转化基金项目(2011G132B100006);农业部“948”项目(2011-G1-21);吉林省财政厅育种项目(201103);吉林省科技发展计划项目(20116030) |
| 摘 要： | 用氟乐灵、拿草特两种抗微管除草剂和秋水仙素在不同处理部位、不同处理时间和浓度条件下对玉米单倍体进行单倍体加倍效率研究。结果表明,3种药剂用浸种法处理玉米单倍体的加倍率以氟乐灵在浓度80μmol/L处理20 h最高(35.7%),用浸芽法处理的加倍率以拿草特在浓度60μmol/L处理24 h最高(24.8%),用浸根法处理的加倍率以氟乐灵在浓度40μmol/L处理15 h最高(16.8%)。因此抗微管除草剂氟乐灵对玉米单倍体加倍效果最好。 |
| 关 键 词： | 玉米 抗微管除草剂 单倍体 加倍效果 |
Doubling Effect of Anti-microtubule Herbicides on the Maize Haploid |
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| Affiliation: | CI Jia-bin1,LI Ji-zhu1,LIU Zhen-ku2,YIN Ri-cheng3,LIANG Xiao-fei4,et al.(1.College of Agronomy,Jilin Agricultural University,Changchun 130118;2.Changchun Seed Management Station,Changchun 130033;3.Yanbian Station Seed Administration,Yanji 133001;4.Jilin Academy of Agricultural Sciences,Changchun 130033,China) |
| Abstract: | Two anti-microtubule herbicides and colchicine were used to double the haploid.Different parts of different processing conditions,processing time and the concentration of maize doubling haploid were treated for researching the double efficiency.The results showed that in three kinds of pharmaceutical treatment,the double rate of immersing seed into trifluralin of the concentration 80 μmol/L for 20 h was the highest(35.7%),the double rate of immersing bud with the pronamide of the concentration 60 μmol/L for 24 h was the highest(24.8%),the double rate of immersing root with the trifluralin of the concentration 40 μmol/L for 15 h is the highest(16.8%).In conclusion,the anti-microtubule herbicide trifluralin on maize doubled haploid was better. |
| Keywords: | Maize Anti-microtubule Haploid Doubling effect |
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