| 蝴蝶兰未受精胚珠离体培养的研究 |
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| 引用本文： | 伍成厚,潘一山,罗开梅,叶秀粦,梁承邺. 蝴蝶兰未受精胚珠离体培养的研究[J]. 园艺学报, 2006, 33(4): 891-894 |
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| 作者姓名： | 伍成厚 潘一山 罗开梅 叶秀粦 梁承邺 |
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| 作者单位： | (漳州师范学院生物系, 福建漳州363000; 中国科学院华南植物园, 广东广州510650; 广州市园林科学研究所,广东广州510405 ) |
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| 基金项目： | 国家自然科学基金资助项目(30170061)，广东省重大科技专项资助项目(2003A2010401)，福建省青年科技人才创新项目资金(2004J052) |
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| 摘 要： | 以蝴蝶兰大孢子母细胞时期的胚珠为外植体进行了离体培养的研究。结果表明, 在VW +BA0.2 mg·L - 1 +NAA 0.2 mg·L - 1 +蔗糖2.0% +植物凝胶0.3%中蝴蝶兰的大孢子母细胞可以发育至二核胚囊; 在VW +BA 0.2 mg·L - 1 +NAA 0.2 mg·L - 1 +蔗糖2.0% +植物凝胶0.3% +椰子汁10%中大孢子母细胞可以发育至四核胚囊或形成胚性细胞团, 这些胚性细胞团有可能发育成单倍体植株。
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| 关 键 词： | 蝴蝶兰 胚珠 胚性细胞团 组织培养 |
| 文章编号： | 0513-353X（2006）04-0891-04 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2005-11-152006-04-27 |
In Vitro Culture of Unfertilized Ovules in Phalaenopsis |
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| Wu Chenghou,Pan Yishan,Luo Kaimei,Ye Xiulin,Liang Chengye. In Vitro Culture of Unfertilized Ovules in Phalaenopsis[J]. Acta Horticulturae Sinica, 2006, 33(4): 891-894 |
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| Authors: | Wu Chenghou Pan Yishan Luo Kaimei Ye Xiulin Liang Chengye |
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| Affiliation: | (Department of Biology, Zhangzhou Normal College, Zhangzhou, Fujian 363000, China; South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; Guangzhou Institute of Landscape and Garden, Guangzhou, Guangdong 510405, China) |
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| Abstract: | The effects of medium composition on the in vitro culture of unfertilized ovules of a Phalaenopsis hybrid were studied. The results showed that the megasporocytes of the hybrid were capable of undergoing meiosis to form bi-nucleate embryo sacs in vitro by ovule cultures on the medium of VW + BA 0.2 mg·L - 1 + NAA 0.2 mg·L - 1 + sucrose 2.0% + phytagel 0.3%. On the medium of VW + BA 0.2 mg·L - 1 +NAA 0.2 mg·L - 1 + sucrose 2.0% + phytagel 0.3% + coconut milk 10% , the megasporocytes would develop into 4-nucleate embryo sacs or embryogenic cell masses. These embryogenic cell masses may develop into haploid plants. |
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| Keywords: | Phalaenopsis Ovule Embryogenic cell masses Tissue culture |
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