鸡传染性法氏囊病毒的分离、纯化与抗血清的制备 |
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引用本文: | 马晓丽 赵晖. 鸡传染性法氏囊病毒的分离、纯化与抗血清的制备[J]. 山东农业科学, 1996, 0(6): 40-42 |
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作者姓名: | 马晓丽 赵晖 |
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摘 要: | 采用鸡胚尿囊液培养传染性法氏囊病毒(IBDV)。将培养病毒的鸡胚尿囊液经高速离心,聚乙二醇(PEG)沉淀、蔗糖垫底超速离心、NaBr密度梯度离心,可获得纯化的鸡传染性法氏囊病毒带。通过透射电子显微镜观察,表明IBDV颗粒呈六角形,直径约60nm,无囊膜,其浮力密度为1.30~1.36g/ml。实验所分离的病毒粒子的平均含量为1.72mg/ml。用这种病毒免疫健康家兔制备抗IBDV的血清,获得了效价在1∶32以上的抗血清,抗血清有较高的纯度和专一性
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关 键 词: | 鸡传染性法氏囊病毒(IBDV) 纯化 抗血清 |
Isolation and Purification of Infectious Bursal Disease Virus and Preparation of Antiserum |
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Abstract: | To get pure infectious bursal disease virus (IBDV) should be done as followed: culture IBDV with allantoic fluid of chicken embryo, centrifuge the fluid at high speed, precipitate it with PEG, putting sucrose at the bottom to centrifuge it again at ultra-high speed, and at last do NaBr density gradient centrifugation. Under JEM-100CX, the virus particle was observed in six-angle shape, free from brain, with diameter being about 60 nm and the buoyant density 1.30-1.36 g/ml, the virus content averaged 1.72 mg/ml. Immuning this virus to rabbit can produce antiserum with 1∶32 of valence, showing high purity and specificity. |
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Keywords: | Chicken IBDV purification antiserum |
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