维生素E、C和HUFA交互作用对中华绒螯蟹生殖性能的影响

通过投喂添加或不添加VE、VC和HUFA的四组实验饲料,经198d饲养实验,以雌蟹的性腺系数、产卵力、孵化率、各组织超氧化物歧化酶(SOD)活性和过氧化产物丙二醛(MDA)含量等为指标,研究这两类营养素交互作用对其生殖性能的影响。结果表明,VE、VC和HUFA对雌蟹生殖性能有显著的影响(P<0.05):在产卵力方面,1组为3790个卵细胞·g-1体重,极显著高于2组(为2317个卵细胞·g-1体重)和3组(为2129个卵细胞·g-1体重)(P<0.01),显著高于4组(为3050个卵细胞·g-1体重)和5组(对照组)(为3010个卵细胞·g-1体重)(P<0.05);而孵化率方面,1组(86.17%)极显著高于2组(36.06%)、3组(29.18%)和4组(25.73%)(P<0.01),也显著高于5组(71.12%)(P<0.05)。1组和2组雌蟹性腺中SOD活性分别为40.09Nu·mL-1和39.87Nu·mL-1,相应地,MDA含量分别为6.87nmol·mL-1和3.33nmol·mL-1;而3组和4组雌蟹,尽管其性腺中SOD活性较高,分别为79.21Nu·mL-1和85.2Nu·mL-1,但由于饲料中抗氧化性VE、VC的缺乏,导致脂质过氧化发生,其MDA含量显著增加(P<0.05),分别为12.65nmol·mL-1和25.18nmol·mL-1。4组雌蟹的产卵力较大,但因VE、VC的缺乏发生脂质过氧化,导致所产的卵质低下,从而影响了卵子的孵化率。结果表明,VE、VC和HUFA都是河蟹保持良好的生殖性能的必需营

Effect of vitamin E and vitamin C, highly unsaturated fatty acids, in broodstock diet on reproduction performance of Eriocheir sinensis

1982day feeding trials were conducted to determine the reciprocal physiological effects on Chinese mitten2handed crabs Eriocheir sinensis female broodstock by feeding with four experiment diets and one control group diet for: diet 1 supplementing HUFA and vitamin E, C; diet 2 supplementing only vitamin E and C; diet 3 supplementing neither HUFA nor vitamin E and C; diet 4 supplementing only HUFA , diet 5 (control group diet) was fresh clam. The results showed that vitamin E, vitamin C and HUFA were essential components in broodstock diets. Fecundity and hatchability of eggs from broodstock crabs fed with diet 1 in which HUFA and vitamin E, C were supplemented were 3790 egg cell·g 1 and 86. 17 % respectively , were the very significantly higher than those of broodstock crabs fed with HUFA2deficient diet like diet 2 and 3 (2317egg cell·g-1, 36.06 % and 2129egg cell ·g-1, 29. 18 %) ( P < 0. 01) , and significantly higher than those of and fecundity and hatchability of eggs (3050 egg cell·g 1 and 25. 73 % in diet 4 treatment) from the crabs fed with diet 4 were also lower than those of control group (3010 egg cell·g 1 and 71. 12 % respectively) ( P < 0. 05) . When the broodstock crabs were fed with vitamin E and C2deficient diets like diet 3 and 4 , lipids of crabs were peroxidized with decreasing SOD activity and increasing MDA content. Meanwhile , the effect of total lipid content , fatty acid composition and vitamin E and C concentrations of experimental diets on those of corresponding eggs , and the lipid peroxidation in eggs are discussed. The results of this study indicate both HUFA and vitamin E , C are indispensable and cooperative to egg developing of crab and must be supplemented in the broodstock crab diet.