抗苯醚菌酯(ZJ0712)小麦白粉病菌突变体的诱导及其生物学特性

经过药剂驯化10代后,从对苯醚菌酯(ZJ0712)敏感的小麦白粉病菌Blumeria graminis f.sp.tritici 3个菌株中获得3个抗药突变体,突变体的抗性指数均大于80,且其抗性能够通过无性繁殖稳定遗传。室内接种试验发现,突变体的致病力与亲本菌株无明显差异。细胞色素b (cyt b)基因片段序列分析发现,突变体cyt b基因的第143位密码子均由敏感菌株的GGT(丙氨酸)突变成了GCT(甘氨酸)。研究结果表明,小麦白粉病菌对苯醚菌酯存在较高的抗性风险,该药剂在使用过程中需注意采取相应的抗性治理措施,以延缓抗性发生。

Induction and Characteristics of Fungicide ZJ0712-resistantBlumeria graminis Mutants

Three fungicide ZJ0712-resistant B.graminis f.sp.tritici (Bgt) mutants Bgt01R,Bgt1-1R and Bgt2-1R were obtained from the sensitive isolates Bgt01,Bgt1-1 and Bgt2-1,respectively by the method of adaptation fungicide for ten generations.The resistance factors of all three mutants to ZJ0712 were greater than 80,and the resistance were stable through asexual reproduction.Inoculation tests showed that the virulence of the mutants was not significantly different from that of their parents.Comparisons of the nucleotide sequence of the partial cytochrome b (cyt b) gene from sensitive isolates and resistant mutants revealed that all three resistant mutants had a single point mutation of GGT (in sensitive isolates) to GCT (in resistant mutants),which caused a change of glycine (Gly) to an alanine (Ala) at codon position 143 (G143A) of the cyt b.The results indicated that the Bgt has a high risk of developing resistance to ZJ0712.In order to delay the development of ZJ0712 resistance in Bgt populations,effective anti-resistance strategies are needed when the fungicide is applied widely in fields.