Occurrence and incidence of viruses infecting green beans in south-eastern Spain |
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Authors: | Eduardo Segundo María P Carmona Elisa Sáez Leonardo Velasco Germán Martín Leticia Ruiz Dirk Janssen Isabel M Cuadrado |
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Institution: | 1.IFAPA Centro La Mojonera,La Mojonera,Spain;2.Laboratorio de Producción y Protección Vegetal de Almería,La Mojonera,Spain;3.Seminis Vegetable Seeds Ibérica,El Ejido,Spain;4.Instituto de Biología Molecular y Celular de Plantas (UPV–CSIC),Universidad Politecnica de Valencia,Valencia,Spain |
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Abstract: | A study was conducted to determine the identity and prevalence of viruses in 455 greenhouses in the main Spanish green bean
growing area. Directed surveys were conducted in 422 crops from 2000–2004 to collect samples from diseased plants displaying
symptoms that could be attributed to viruses. The samples were analysed to detect any virus by means of dsRNA extraction,
mechanical inoculation to test plants, as well as ELISA and/or RT-PCR tests to detect potyviruses, geminiviruses and viruses
previously known to infect beans in Spain. Random surveys were conducted in the years 2002 and 2005 (in 21 and 12 greenhouses,
respectively) to study the actual incidence of known viruses in the area. Symptoms were recorded in 23,108 plants from which
664 plants were collected and analysed by ELISA or RT-PCR. The results of the directed surveys showed that all the analyzed
crops carried the cryptic virus Phaseolus vulgaris endornavirus (PVuV), whereas phytopathogenic viruses appeared in smaller percentages of the crops: Tomato yellow leaf curl virus (TYLCV) 20.4%, Southern bean mosaic virus (SBMV) 9.0%, Tomato spotted wilt virus (TSWV) 4.0%, and the new species Bean yellow disorder virus (BnYDV) that broke out in 2004 with occurrence values higher than 34.3% that year. From 2000–2004 an important decrease in
TYLCV was observed, along with a slight increase in SBMV and a consistently low occurrence of TSWV. The results of the random
surveys confirmed the increased occurrence of virus detected during the directed surveys, and furthermore demonstrated the
percentage of incidence for each virus. |
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Keywords: | Phaseolus vulgaris Virus incidence Greenhouse |
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