首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Occurrence and incidence of viruses infecting green beans in south-eastern Spain
Authors:Eduardo Segundo  María P Carmona  Elisa Sáez  Leonardo Velasco  Germán Martín  Leticia Ruiz  Dirk Janssen  Isabel M Cuadrado
Institution:1.IFAPA Centro La Mojonera,La Mojonera,Spain;2.Laboratorio de Producción y Protección Vegetal de Almería,La Mojonera,Spain;3.Seminis Vegetable Seeds Ibérica,El Ejido,Spain;4.Instituto de Biología Molecular y Celular de Plantas (UPV–CSIC),Universidad Politecnica de Valencia,Valencia,Spain
Abstract:A study was conducted to determine the identity and prevalence of viruses in 455 greenhouses in the main Spanish green bean growing area. Directed surveys were conducted in 422 crops from 2000–2004 to collect samples from diseased plants displaying symptoms that could be attributed to viruses. The samples were analysed to detect any virus by means of dsRNA extraction, mechanical inoculation to test plants, as well as ELISA and/or RT-PCR tests to detect potyviruses, geminiviruses and viruses previously known to infect beans in Spain. Random surveys were conducted in the years 2002 and 2005 (in 21 and 12 greenhouses, respectively) to study the actual incidence of known viruses in the area. Symptoms were recorded in 23,108 plants from which 664 plants were collected and analysed by ELISA or RT-PCR. The results of the directed surveys showed that all the analyzed crops carried the cryptic virus Phaseolus vulgaris endornavirus (PVuV), whereas phytopathogenic viruses appeared in smaller percentages of the crops: Tomato yellow leaf curl virus (TYLCV) 20.4%, Southern bean mosaic virus (SBMV) 9.0%, Tomato spotted wilt virus (TSWV) 4.0%, and the new species Bean yellow disorder virus (BnYDV) that broke out in 2004 with occurrence values higher than 34.3% that year. From 2000–2004 an important decrease in TYLCV was observed, along with a slight increase in SBMV and a consistently low occurrence of TSWV. The results of the random surveys confirmed the increased occurrence of virus detected during the directed surveys, and furthermore demonstrated the percentage of incidence for each virus.
Keywords:Phaseolus vulgaris            Virus incidence  Greenhouse
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号