| 虎眼万年青的组织培养与快速繁殖 |
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| 引用本文： | 彭世勇,关丽霞,佟岩,孙营. 虎眼万年青的组织培养与快速繁殖[J]. 辽宁农业职业技术学院学报, 2006, 8(3): 6-7 |
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| 作者姓名： | 彭世勇 关丽霞 佟岩 孙营 |
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| 作者单位： | 辽宁农业职业技术学院,辽宁,营口,115009;辽宁农业职业技术学院,辽宁,营口,115009;辽宁农业职业技术学院,辽宁,营口,115009;辽宁农业职业技术学院,辽宁,营口,115009 |
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| 摘 要： | 以虎眼万年青的鳞片为外植体,研究不同接种方式、NAA和6-BA不同配比对诱导小鳞茎、芽苗增殖及生根的影响。结果表明,虎眼万年青鳞片以内表面向上接种的方式最易诱导出小鳞茎,且以接种于培养基MS0 NAA1.0 BA1.5上的诱导率为最高,达84.0%;增殖效果最佳的培养基为MS0 NAA0.5 6-BA1.0,增殖率为150%;促进生根和根系素质较好的培养基为1/2MS0 NAA0.5 IBA0.2,生根率达76.1%。
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| 关 键 词： | 虎眼万年青 鳞片 外植体 小鳞茎 增殖 |
| 文章编号： | 1671-0517(2006)03-0006-02 |
| 收稿时间： | 2006-07-18 |
| 修稿时间： | 2006-07-18 |
Tissue Culture and Rapid Propagation of [Rohdea japonica(Thunb.)Roth.] |
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| PENG Shi-yong,GUAN Li-xia,TONG Yan,SHUN Ying. Tissue Culture and Rapid Propagation of [Rohdea japonica(Thunb.)Roth.][J]. Journal of Liaoning Agricultural Vocation-Technical College, 2006, 8(3): 6-7 |
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| Authors: | PENG Shi-yong GUAN Li-xia TONG Yan SHUN Ying |
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| Affiliation: | Liaoning Agricultural College, Yingkou 115009, China |
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| Abstract: | Effecting factors of different construction of NAA and 6-BA on bulblet inducement,test-tube plantlet reproduction and root growth and different method to inoculate were studied by use of scale leaf of [Rohdea japonica(Thunb.)Roth.].The result indicated that the ascending inner epidermis of scale leaf to induce bulblet was the optimum inoculating method and the inducing rate was up to 84% on culture medium MS_0 NAA1.0 BA1.5.The medium had better reproductive effect was MS_0 NAA0.5 6-BA1.0 and the reproductive percentage was up to 150%.In addition,the medium to accelerate root growth and increase root system quality was 1/2MS_0 NAA0.5 IBA0.2 and the rate of toot growth was up to 76.1%. |
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| Keywords: | [Rohdea japonica(Thunb.)Roth.] Scale Leaf Explant Bulblet Reproduction Root Growth |
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