| 水稻抗除草剂基因bar的转育研究 |
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| 引用本文： | 王才林,赵凌,宗寿余,吕川根,邹江石,何小兰,朱为民.水稻抗除草剂基因bar的转育研究[J].作物学报,2002,28(3):305-309. |
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| 作者姓名： | 王才林 赵凌 宗寿余 吕川根 邹江石 何小兰 朱为民 |
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| 作者单位： | 江苏省农业科学院,江苏南京,210014 |
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| 基金项目： | 江苏省高技术研究计划项目 (BG2 0 0 13 0 4),江苏省应用基础研究项目 (BJ2 0 0 0 0 41),中国水稻科学基金项目 (0 0 0 3 2 15 ) |
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| 摘 要： | 本研究以转bar基因水稻"HR"为供体,两系法亚种间杂交稻优势组合"两优培九"的恢复系"9311"为受体,经过3次回交转育成了对Basta除草剂具有抗性的9311近等基因系"9311HR",经PCR分析确认已导入bar基因.比较试验表明,9311HR及其与"培矮64S"配制的杂种的产量、米质、抗性等主要农艺性状分别与9311和两优培九十分相似.表明通过回交将
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| 关 键 词： | 水稻 抗除草剂 bar基因 回交 转育 |
| 收稿时间： | 2001-07-11 |
| 修稿时间： | 2001年7月11日 |
Transfer of Herbicide Resistant Gene bar into New Rice Variety by Backcrossing |
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| WANG Cai,Lin,ZHAO Ling,ZONG Shou,Yu,LU Chuan,Gen,ZOU Jiang,Shi,HE Xiao,Lan,ZHU Wei,Min.Transfer of Herbicide Resistant Gene bar into New Rice Variety by Backcrossing[J].Acta Agronomica Sinica,2002,28(3):305-309. |
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| Authors: | WANG Cai Lin ZHAO Ling ZONG Shou Yu LU Chuan Gen ZOU Jiang Shi HE Xiao Lan ZHU Wei Min |
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| Abstract: | A new transgenic rice "9311HR" resistant to herbicide "Basta" was bred by transferring the bar gene from the donor, a genetically modified organism (GMO) "HR" obtained by biolistic method, into the breeding line "9311", a restorer of the hybrid "Liangyoupeijiu" by successive backcrossing. PCR analysis confirmed that "9311HR" showed positive reaction of the bar gene. "9311HR" and its hybrid with TGMS line "Peiai 64S" were very similar to "9311" and "Liangyoupeijiu" respectively in yield performance, grain quality, resistance to diseases and other agronomic traits. The results confirmed that to transfer bar gene into other variety by backcrossing is a simple and effective approach to getting a new rice variety resistant to herbicide "Basta". |
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| Keywords: | Rice ( Oryza sativa L ) Herbicide resistance bar gene Backcross Transfer |
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