牛羊源沙门氏菌在某定点屠宰场和农贸市场的污染分布与耐药性调查

【目的】 研究新疆地区某定点屠宰场和农贸市场中牛羊源沙门氏菌的污染和耐药性。【方法】 在新疆乌鲁木齐市某定点屠宰场、库尔勒市某定点屠宰场和农贸市场采集的胴体拭子、肉样、加工用具拭子841份,采用国家标准方法GB 4789.4-2016进行沙门氏菌的分离鉴定,并进行17种药敏纸片的耐药性调查。【结果】 共分离出47株沙门氏菌属,分离率为5.59%。在屠宰场中沙门氏菌污染程度依次为:胴体拭子＜加工用具拭子＜肉样。而在农贸市场中沙门氏菌污染程度依次为:加工用具拭子＜肉样＜胴体拭子。对17种抗菌药物均存在不同程度的耐药,耐药率为2.13%～59.57%。多重耐药沙门氏菌数量较多,其中有1株沙门氏菌耐12种抗菌药。【结论】 市场中沙门氏菌的多重耐药比屠宰场的严重。沙门氏菌在屠宰和市场环节均存在污染,且大多数都是耐药菌株。

Pollution Distribution and Drug Resistance of Salmonella from Cattle and Sheep in Designated Slaughterhouse and Trade Markets

【Objective】 To investigate the contamination and drug resistance of Salmonella from cattle and sheep in designated slaughterhouse and trade markets in Xinjiang. 【Method】 A total of 841 carcass swabs, meat swabs and processing gear swabs were collected in Urumqi designated slaughterhouses, Korla designated slaughterhouses and trade markets. The national standard method GB 4789.4-2016 was used to isolate and identify Salmonella. The drug resistance of 17 kinds of drug-sensitive paper was investigated. 【Result】 In our study, 47 Salmonella strains were isolated, and the isolation rate was 5.59%. The contamination degree of Salmonella in designated slaughterhouse was as follows: carcass swab < processing tool swab < meat sample. The contamination degree of Salmonella in trade markets was as follows: processing tool swab < meat sample < carcass swab. There were different degrees of resistance to 17 kinds of antibacterial drugs. The drug resistance rate was 2.13% -59.57%. Only seven strains of Salmonella were sensitive. The number of multidrug-resistant Salmonella was more. Only one strain of Salmonella was resistant to 12 antibiotics. 【Conclusion】 Salmonella multidrug resistance in the market is more severe than in slaughterhouses and it is contaminated in slaughtering and marketing, and most of the strains are drug-resistant ones.