| 藏獒精液冷冻过程中pH值及甘油对其活力的影响 |
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| 引用本文： | 辛国省,张勇,龙瑞军,赵兴绪.藏獒精液冷冻过程中pH值及甘油对其活力的影响[J].经济动物学报,2007,11(4):202-205. |
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| 作者姓名： | 辛国省 张勇 龙瑞军 赵兴绪 |
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| 作者单位： | 兰州大学草地农业科技学院,兰州,730000;兰州市爱心宠物医院,兰州,730070;甘肃农业大学动物医学院,兰州,730070;兰州大学草地农业科技学院,兰州,730000 |
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| 摘 要： | 为探讨pH值和甘油对藏獒精液冷冻保存前后精子活力的影响,筛选6条种公藏獒在其繁殖季节(9~12月份)以不同pH值梯度(5.5,6.0,6.5,7.0,7.5)稀释液和不同甘油平衡时间(10,20,30,40,50,60,70,80,90 min)对藏獒精液进行冷冻,检查精子活力变化。结果表明,在藏獒精液冷冻过程中,pH值为6.5的稀释液中精子在冷冻前后有较高的活力;甘油平衡时间对藏獒精液活力有较大影响,冻前精子活力随甘油作用时间而下降,在甘油平衡时间为20 min所得藏獒冻后精子活力效果较好。
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| 关 键 词： | 藏獒 精子 活力 pH值 平衡时间 |
| 文章编号： | 1007-7448（2007）04-0202-04 |
| 收稿时间： | 2007-05-07 |
| 修稿时间： | 2007年5月7日 |
Effect of Extender pH and Glycerin on Motility of Tibetan Mastiff Frozen Semen |
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| XIN Guo-sheng,ZHANG Yong,LONG Rui-jun,ZHAO Xing-xu.Effect of Extender pH and Glycerin on Motility of Tibetan Mastiff Frozen Semen[J].Journal of Economic Animal,2007,11(4):202-205. |
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| Authors: | XIN Guo-sheng ZHANG Yong LONG Rui-jun ZHAO Xing-xu |
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| Abstract: | The effect of different extender pH(5.5,6.0,6.5,7.0,7.5) and different balance time(10,20,30,40,50,60,70,80,90 min)of Glycerin on the motility of frozen sperm of Tibetan Mastiff in their reproduction season(Sep-Dec) was studied in the experiment.The results showed that the motility of sperm was higher in ph 6.5 extender than in the others in the condition of 4℃.The balance time of glycerin affected the motility of sperm greatly,and the balance time of 20 min was better for motility of frozen sperm than that of others. |
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| Keywords: | Tibetan Mastiff semen motility pH time of balance |
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