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水提醇沉法提取油茶饼粕多糖的响应面优化
引用本文:张盟雨,王静,张应中,王明怀,徐煲铧,唐旭晓. 水提醇沉法提取油茶饼粕多糖的响应面优化[J]. 广东林业科技, 2019, 35(5): 1-6
作者姓名:张盟雨  王静  张应中  王明怀  徐煲铧  唐旭晓
作者单位:广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520;广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520;广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520;广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520;广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520;广东省森林培育与保护利用重点实验室 / 广东省林业科学研究院,广东 广州,510520
基金项目:广东省林业科技创新项目(2017KJCX003)、广东省级科技计划项目(2016A040401008)、广东省级科技计划项目(2015B020202002)
摘    要:为优化水提醇沉法提取油茶 (Camellia oleifera) 饼粕多糖工艺条件,在单因素试验基础上,选择料液比、提取温度、提取时间为自变量,油茶多糖提取率为响应值,利用 Box-Behnken 中心组合试验和响应面分析法,研究各自变量交互作用及其对油茶多糖提取率的影响。结果表明,油茶多糖提取最佳提取工艺为料液比 1:25(g/mL)、提取时间 78 min、提取温度 60 ℃,在此条件下油茶多糖提取率为(11.29 ± 0.20)%,与预测值偏差 0.18 个百分点,因此优化后回归方程对预测水提醇沉法制备油茶多糖的提取率分析具有可靠性,三因素中料液比具有显著性影响。

关 键 词:油茶饼粕多糖  水提醇沉法  提取率  响应面分析
收稿时间:2019-07-15
修稿时间:2019-09-18

Optimization of Water Extraction and Alcohol Precipitation Method for Preparation of Camellia oleifera Cake Polysaccharides by Response Surface Analysis
zhangmengyu,wangjing,zhang yingzhong,wangminghuai,xubaohua and tangxiaoxu. Optimization of Water Extraction and Alcohol Precipitation Method for Preparation of Camellia oleifera Cake Polysaccharides by Response Surface Analysis[J]. Forestry Science and Technology of Guangdong Province, 2019, 35(5): 1-6
Authors:zhangmengyu  wangjing  zhang yingzhong  wangminghuai  xubaohua  tangxiaoxu
Affiliation:Guangdong Academy of Forestry,Guangdong Academy of Forestry,Guangdong Academy of Forestry,Guangdong Academy of Forestry,Guangdong Academy of Forestry,Guangdong Academy of Forestry
Abstract:In order to optimize the water extraction and alcohol precipitation method for preparation of Camellia oleifera cake polysaccharides, on the basis of single factor experiment, the ratio of material to liquid, extraction temperature and extraction time were chosen as independent variables, while the extraction rate of C. oleifera polysaccharides as corresponding values. With the Box-Behnken center combined test and response surface analysis, the interaction of each independent variable and its effect on the extraction rate of C. oleifera polysaccharide were studied. The results showed that the optimum for C. oleifera polysaccharides preparation by water extraction and alcohol precipitation were 1:25(g/mL) of the ratio of solid to liquid, 78 min of the extraction time, and 60 °C of the extraction temperature. Under these conditions, the extraction rate of C. oleifera polysaccharides was (11.29 ±0.20)%, with a deviation of 0.18 percentage points from the predicted value. Therefore, this optimized regression equation has the reliability of predicting the extraction rate of C. oleifera polysaccharides by the water extraction and alcohol precipitation method, and the ratio of solid to liquid has a significant effect in the three factors.
Keywords:Camellia oleifera cake polysaccharides   water extraction and alcohol precipitation   extraction rate   response surface methology
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