乐山金口河区板厂坪蜜环菌的分离及系统发育分析

蜜环菌作为天麻重要共生菌能为天麻的生长提供营养。了解区域性蜜环菌的多样性有助于选育优良的土著蜜环菌,进而提高区域性天麻的产量和质量。本文应用纯培养技术和基于ITS基因序列的系统发育分析,对金口河板厂坪天麻种植区分离的21份蜜环菌进行了多样性研究。结果表明,21株蜜环菌划分为6个形态类群；系统发育分析显示分离菌株与Armillaria 同源性最近,相似性都在99% 以上,主要分布在Armillaria gallica、Armillaria sinapina和Armillaria mellea 3个种内。

Isolation and Phylogenetic Analysis of Armillaria from Jinkouhe Banchangping Leshan

As an important symbiotic bacterium of Gastrodia elata, Armillaria can provide nutrition for the growth of Gastrodia elata. Understanding the diversity of regional honey fungus will help to select excellent Armillaria, and thus increase the yield and quality of Gastrodia elata. In this paper, the pure culture technique and phylogenetic analysis based on ITS gene sequence were used to study the diversity of 21 Armillaria isolated from the Gastrodia elata planting area of Jinkouhe Banchangping. The results showed that 21 strains of Armillaria were divided into 6 morphological groups. The phylogenetic analysis showed that the isolates had the closest homology with Armillaria, and the similarity was over 99%, mainly distributed in Armillaria gallica, Armillaria sinapina and Armillaria mellea.