棉花枯萎病致病菌定量检测的研究

选择镰刀菌用驹田或植选1号培养基,在米饭培养基上划分镰刀菌种群,用试管法或盆栽作致病性测定,以检测土壤中棉花枯萎病致病菌。试验结果表明,该致病菌主要在奶油色、淡青莲及深紫色菌群中,分别占21.2%,11.5%,4.5%。陕西杨陵(1985)棉枯萎病病圃(发病株率85%以上),播种前10公分土层内每克干土含菌量为2236～2576个菌落。使用这一定量检测程序比单独使用选择性培养基提高了精确度,方法简便易行。

STUDIES OF THE QUANTITATIVE TEST OF Fusarium oxysporum f.sp.Vasinfectum Snyd.et Hans.IN SOIL

Rice medium was used to identify the species groups of genus of Fusarium fungi selected by Komada or No.1 selective culture medium from soil.The glass tube or the pot cultivation method was also used to determine the pathogenicity of pathogens so as to detect Fusarium wilt of cotton in soil.Experimental results indicated that Fusarium oxysporum f.sp vasinfectum mainly occured among the cream,light purple and dark purple species groups at the rate of 21.2%,11.5% and 4.5% respectively,and that before sowing in the upper 10 cm of soil layer there might be 2236-2576 colonies of pathogens per gram of dry soil in the cotton wilt disease nursery(more than 85% of wilted plants) in Yangling area,Shaanxi.The detective precision by using this quantitative detective procedure was much more improved that by solely using the selective culture medium.Also,this method is simple and practical.