| 大熊猫皮肤组织酶解分离效果研究 |
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| 引用本文： | 张明,郑鸿培,侯蓉,朱庆,张志和,刘玉良,刘磊,鲜红,李骏. 大熊猫皮肤组织酶解分离效果研究[J]. 四川农业大学学报, 2004, 22(1): 75-78 |
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| 作者姓名： | 张明 郑鸿培 侯蓉 朱庆 张志和 刘玉良 刘磊 鲜红 李骏 |
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| 作者单位： | 四川农业大学,动物科技学院,四川,雅安,625014;成都大熊猫繁育研究基地,四川,成都,610081 |
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| 基金项目： | 成都大熊猫繁育基金会资助 |
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| 摘 要： | 大熊猫皮肤组织块在 2 0 0IU/mL胶原蛋白酶Ⅰ和 0 2 5 %胰蛋白酶中 ,分别在 37℃、4℃下进行酶解的 4个方案中 ,以在 2 0 0IU/mL胶原蛋白酶Ⅰ ,在 4℃下酶解 4 8～ 72h的酶解效果最好 ,活细胞的得率达到 4 6 0 4× 10 6/g ,且胶原蛋白酶Ⅰ酶解得到的细胞以成纤维细胞为主
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| 关 键 词： | 大熊猫 成纤维细胞 胶原蛋白酶Ⅰ 胰蛋白酶 |
| 文章编号： | 1000-2650(2004)01-0075-04 |
| 修稿时间： | 2003-09-25 |
Studies on Enzyme Digestion of Cutaneous Tissue of Giant Panda (Ailuropoda Melanoleuca) |
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| ZHANG Ming,ZHENG Hong-pei,HOU Rong,ZHU Qing,ZHANG Zhi-he,LIU Yu-liang,LIU Lei,XIAN Hong,LI Jun. Studies on Enzyme Digestion of Cutaneous Tissue of Giant Panda (Ailuropoda Melanoleuca)[J]. Journal of Sichuan Agricultural University, 2004, 22(1): 75-78 |
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| Authors: | ZHANG Ming ZHENG Hong-pei HOU Rong ZHU Qing ZHANG Zhi-he LIU Yu-liang LIU Lei XIAN Hong LI Jun |
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| Affiliation: | ZHANG Ming1,ZHENG Hong-pei1,HOU Rong2,ZHU Qing1,ZHANG Zhi-he2,LIU Yu-liang2,LIU Lei1,XIAN Hong1,LI Jun1 |
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| Abstract: | In the paper, we studied that custaneous tissue of giant panda was digested with collagenase-Ⅰ (200?IU/mL) or trypsin (0.25%) under 37?℃ or 4?℃. In the 4 treatments, the effect shows: the method of enzyme digestion which was treated with collagenase-Ⅰ (200?IU/mL) under 4℃ for 48～72?h was the best in all methods and the rate of living cells reached 4.604×106/g. In addition, the fibroblast cells was main cells in the test treated with collagenase-Ⅰ (200?IU/mL). |
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| Keywords: | giant panda fibroblast collagenase type Ⅰ trypsin |
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