| 野生与栽培药材麻花秦艽的HPLC/UV指纹图谱研究 |
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| 引用本文： | 纪兰菊,马玉花,陈桂琛.野生与栽培药材麻花秦艽的HPLC/UV指纹图谱研究[J].安徽农业科学,2006,34(10):2165-2167. |
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| 作者姓名： | 纪兰菊 马玉花 陈桂琛 |
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| 作者单位： | 中国科学院西北高原生物研究所,青海,西宁,810001;中国科学院西北高原生物研究所,青海,西宁,810001;中国科学院西北高原生物研究所,青海,西宁,810001 |
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| 基金项目： | 青海省重点科技攻关项目(2004-N-142)，国家中西部专项(2001BA901A47) |
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| 摘 要： | 采用反相高效液相色谱—二极管阵列的检测方法,分析了不同产地11批麻花秦艽药材,建立麻花秦艽药材的指纹图谱,进行野生药材与栽培药材的对比研究。结果表明:最佳条件可较全面的反映麻花秦艽药材的主要成分,栽培药材与野生药材相似度较好。
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| 关 键 词： | 藏药 麻花秦艽 指纹图谱 RP-HPLC/UV |
| 文章编号： | 0517-6611（2006）10-2165-03 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006-02-20 |
Study on the Chromatographic Fingerprint of Tibet Berb Gentiana straminea Maxim by HPLC/UV |
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| JI Lan-ju et al.Study on the Chromatographic Fingerprint of Tibet Berb Gentiana straminea Maxim by HPLC/UV[J].Journal of Anhui Agricultural Sciences,2006,34(10):2165-2167. |
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| Authors: | JI Lan-ju |
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| Institution: | Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining,Qinghai 810001 |
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| Abstract: | The chromatographic fingerprint of Gentiana straminea Maxim has been set up by high performance liquid chromatography-diode-array detection method for eleven batches herbs collected in different districts of Qinghai-Tibet Plateau compare the chromatogram of wild angplanted samples.The analysis was performed on a Kromasil C18(250 mm×4.60 mm,5 μm)eluted with methanol and 0.02 % aqueous phosphoric acid as mobile phase and with UV detection at 254 nm.A general fingerprint acquaintance of main constituents in Gentiana straminea Maxim can be obtained by HPLC method,and the similarity is excellent between wild and plented samples,which provides scientific indexes for quality of Gentiana straminea Maxim. |
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| Keywords: | Tibet herb Gentiana straminea Maxim Chromatographic fingerprint RP-HPLC |
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