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The Use of Ovule Culture in Reciprocal Hybridization between B. campestris L. and B. oleracea L.
Authors:E. Diederichsen   M. D. Sacristan
Affiliation:Institut für Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, D-14195 Berlin, Germany
Abstract:Ovule culture was used to overcome postzygotic interspecific incompatibility in reciprocal crosses between Brassica campestris and B. oleracea. Hybrid plants were obtained from 82 % of all reciprocal pollinations, representing 91 % of the attempted combinations. Comparisons of the embryo culture and the ovule culture indicated a significantly higher germination rate after ovule culture.
Keywords:B. oleracea    B. campestris    B. napus    interspecific crosses    ovule culture
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