The Use of Ovule Culture in Reciprocal Hybridization between B. campestris L. and B. oleracea L. |
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Authors: | E. Diederichsen M. D. Sacristan |
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Affiliation: | Institut für Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, D-14195 Berlin, Germany |
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Abstract: | Ovule culture was used to overcome postzygotic interspecific incompatibility in reciprocal crosses between Brassica campestris and B. oleracea. Hybrid plants were obtained from 82 % of all reciprocal pollinations, representing 91 % of the attempted combinations. Comparisons of the embryo culture and the ovule culture indicated a significantly higher germination rate after ovule culture. |
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Keywords: | B. oleracea B. campestris B. napus interspecific crosses ovule culture |
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