硝磺草酮降解菌HZ-2制剂固定化载体材料性能的比较

【目的】研究改性蔗渣MSB、MSB-Mo、MSB-Cl对硝磺草酮降解菌HZ-2的降解能力的影响。【方法】以这3种改性蔗渣和活性炭为固定化载体,对固定化后菌制剂的降解性能进行研究。【结果】降解菌制剂培养48 h后,D600 nm均较高,表明MSB、MSB-Mo、MSB-Cl及活性炭均具备较强的固菌能力;培养3 d后,以改性蔗渣为载体的固菌制剂对LB培养基中硝磺草酮的降解率高于85%,显著优于活性炭,其中改性蔗渣MSB-Mo效果突出,降解率达96.35%。添加葡萄糖辅料7 d后,这3种改性蔗渣对土壤中硝磺草酮的降解率均高于99%。【结论】改性蔗渣MSB-Mo是固定降解菌株的最佳新型生物载体材料。

Pexformance comparison of different carrier materials for immobilization of mesotrione-degrading strain HZ-2

Objective] To evaluate the effect of modified bagasse (MSB, MSB-Mo and MSB-CI) on the immobilization of mesotrione-degrading bacteria strain HZ-2 .[Method]Three types of modified bagasse and activated carbon were selected as the carrier candidates for the bacterial immobilization , and the deg-radation abilities of immobilized composites were examined .[Result]After 48 hours culture , the high D600 nm values indicated that modified bagasse ( MSB, MSB-Mo and MSB-CI ) and activated carbon had strong abilities to immobilize bacteria .More than 85%of mesotrione in LB medium was degraded using modified bagasse as a carrier after 3 days of culture , which was higher than the results of activated car-bon .MSB-Mo composite had the best degradation performance with a degradation rate of 96.35%.More-over ,mesotrione in soil was degraded over 99%after 7 days by adding glucose as support nutrient to the modified bagasse .[Conclusion] Modified bagassse MSB-Mo is the most available carrier material , which demonstrates the highest potential of being a new biological carrier material for immobilization of mesotri -one-degrading bacteria strain .