| EGCG抑制PC12凋亡的细胞生物学研究 |
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| 引用本文： | 沈生荣,金超芳,赵保路.EGCG抑制PC12凋亡的细胞生物学研究[J].茶叶科学,2001,21(2):148-152,111. |
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| 作者姓名： | 沈生荣 金超芳 赵保路 |
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| 作者单位： | 浙江大学农业与生物技术学院茶学系,浙江大学农业与生物技术学院茶学系,中国科学院生物物理研究所分子细胞生物学研究室 浙江杭州310029 ,浙江杭州310029 ,北京100101 |
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| 摘 要： | 以神经毒素 6_OHDA诱导PC12细胞凋亡长期被用作帕金森氏病 (PD)研究模型。由于氧应激在PD发病中起重要作用 ,在 6_OHDA处理前提前 3 0min加入不同剂量的保护剂EGCG ,对 6_OHDA造成的PC12细胞损伤有一定的保护作用 ;流式细胞术检测细胞凋亡率 ,结果表明EGCG表现出剂量依赖的保护作用 ,且在 2 0 0 - 4 0 0 μM时保护作用最佳 ;荧光结果也表明EGCG保护效果明显
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| 关 键 词： | 凋亡 儿茶素 PC12细胞 6_羟多巴胺 帕金森氏病 抗氧化 |
| 文章编号： | 1000-369X(2001)02-0148-05 |
The Cellular Biological Investigation on the Inhiboratory Activity of EGCG on Apoptosis of PC12 Cells in vitro |
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| SHEN Sheng_rong ,JIN Chao_fang ,ZHAO Bao_lu.The Cellular Biological Investigation on the Inhiboratory Activity of EGCG on Apoptosis of PC12 Cells in vitro[J].Journal of Tea Science,2001,21(2):148-152,111. |
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| Authors: | SHEN Sheng_rong JIN Chao_fang ZHAO Bao_lu |
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| Institution: | SHEN Sheng_rong 1,JIN Chao_fang 1,ZHAO Bao_lu 2 |
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| Abstract: | The biological activities of (-)_Epigallocatechins gallate (EGCG) were investigated by observing the range of 6_OHDA_induced apoptosis of PC12 cells in the Parkinson's disease in vitro model. Measured by MTT assay, 6_OHDA inhibited cell viability in time_ and concentration_dependent manner. When PC12 cells were pretreated with EGCG for 30min before exposured to 250 μM 6_OHDA, results showed that EGCG had obvious concentration_dependent protective effects in the range of 50-400 μM; 200-400 μM EGCG showed best effects. In addition, typical apoptotic characteristics of PC12 cells were determined by fluoresence microscopy, after the cells treated with 250 μM 6_OHDA for 24 h or pretreated with EGCG before it. Results showed preincubation with 200-400μM EGCG led to significant inhibitory effects against PC12 cell apoptosis. |
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| Keywords: | Apoptosis Catechins EGCG PC12 cells 6-OHDA Parkinson's disease Antioxidant |
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