| 日本蟳精子超低温冷冻保存技术的研究 |
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| 引用本文： | 许星鸿,阎斌伦,徐加涛,徐国成,浦寅芳,许建和. 日本蟳精子超低温冷冻保存技术的研究[J]. 水产科学, 2010, 29(10) |
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| 作者姓名： | 许星鸿 阎斌伦 徐加涛 徐国成 浦寅芳 许建和 |
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| 作者单位： | 淮海工学院,江苏省海洋生物技术重点建设实验室,江苏,连云港,222005 |
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| 基金项目： | 江苏省海洋生物技术重点建设实验室开放课题(Z2007HS006); 连云港市科技发展计划项目(SF0705) |
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| 摘 要： | 以精子存活率作为评价指标,采用两步降温法研究了稀释剂、抗冻保护剂和预冷时间对日本蟳精子超低温冷冻保存效果的影响。结果表明:以采用稀释剂II、15%二甲基亚砜(DMSO)作为抗冻保护剂的保存效果最佳。在液氮中保存24 h后,精子存活率可达83.76%,保存一年后可达73.81%。精液的第一次预冷时间以25 min为宜。
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| 关 键 词： | 日本蟳 精子 超低温保存 |
Cryopreservation of Spermatozoa in Japanese Swimming Crab Charybdis japonica |
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| XU Xing-hong,YAN Bin-lun,XU Jia-tao,XU Guo-cheng,PU Yin-fang,XU Jian-he. Cryopreservation of Spermatozoa in Japanese Swimming Crab Charybdis japonica[J]. Fisheries Science, 2010, 29(10) |
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| Authors: | XU Xing-hong YAN Bin-lun XU Jia-tao XU Guo-cheng PU Yin-fang XU Jian-he |
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| Affiliation: | XU Xing-hong,YAN Bin-lun,XU Jia-tao,XU Guo-cheng,PU Yin-fang,XU Jian-heJiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China) |
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| Abstract: | The effects of sperm diluents,cryoprotectants and prefreezing times on viability of the spermatozoa in Japanese swimming crab Charybdis japonica were detected during spermatozoa two-step freezing cryopreservation.The results showed that DMSO was found to be better cryoprotectant than glycerin for cryopreservation of the spermatozoa and sperm diluent with the optimum 15% DMSO.The sperm viability was 83.76% 24 hours after preservation and 73.81% one year after preservation in liquid nitrogen.The prefreezing t... |
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| Keywords: | Charybdis japonica spermatozoon cryopreservation |
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