Effects of Vermicompost and Vermiwash on Plant,Phenolic Content,and Anti-oxidant Activity of Mexican Pepperleaf (Piper auritum Kunth) Cultivated in Phosphate Rock Potting Media

The aim of this investigation was to determine the effect of vermicompost, vermiwash, and phosphate rock on plant, total phenols, flavonoids, and anti-oxidant activity in Piper auritum Kunth leaves. P. auritum plants were obtained from cuttings and were planted according to the Box-Behnken experimental design with three repetitions at the central point. The factors and levels were vermicompost (10, 20, and 30 g plant^?1), vermiwash (5, 10, and 15 mL plant^?1), and phosphate rock (1, 2, and 3 g plant^?1). Plant growth parameters (plant height, stem diameter, leaves number) and chlorophyll content were measured 1 month after treatment applications. Total phenols, total flavonoids, and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity was measured after 4 months. Vermicompost, vermiwash, and phosphate rock had no statistically significant effect on plant growth. Plant height, stem diameter, leaves number, chlorophyll, innermost number, fresh weight stem, fresh weight leaves, fresh weight root, dry weight stem, dry weight leaves, and dry weight root were not different among treatments. Total phenolic compounds were statistically affected for both vermiwash and phosphoric rock (p < 0.05) and the anti-oxidant activity decreased by vermicompost addition. The application of 15 mL plant^?1 vermiwash, 1 g phosphate rock, and 20 g vermicompost plant^?1 increased the total phenol content.