| AllGlo实时荧光PCR快速检测6种按实蝇 |
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| 引用本文： | 徐浪,余道坚,李建光,王银竹,陈志粦.AllGlo实时荧光PCR快速检测6种按实蝇[J].植物检疫,2010(5). |
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| 作者姓名： | 徐浪 余道坚 李建光 王银竹 陈志粦 |
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| 作者单位： | 深圳出入境检验检疫局;北京出入境检验检疫局;中山大学昆虫学研究所有害生物控制与利用国家重点实验室; |
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| 基金项目： | 国家质检总局项目(2007IK249) |
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| 摘 要： | 按实蝇属(双翅目:实蝇科)是国际关注的重要果蔬害虫,也是我国进境植物检疫性有害生物。按实蝇种类多,形态学方法很难进行准确鉴定。本研究首次利用mtDNACOI基因设计了6条特异性探针,应用AllGlo实时荧光PCR方法实现了南美按实蝇、墨西哥按实蝇、西印度按实蝇、印加按实蝇、中美按实蝇和A.chiclayae Greene等6种重要的按实蝇的快速种类鉴定。
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| 关 键 词： | 按实蝇 AllGlo实时荧光PCR 鉴定 |
Detection of six Anastrepha spp. (Diptera:Tephritidae) by AllGlo fluorescence real-time PCR |
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| Xu Lang,Yu Daojian ,Li Jianguang,Wang Yinzhu,Chen Zhilin.Detection of six Anastrepha spp. (Diptera:Tephritidae) by AllGlo fluorescence real-time PCR[J].Plant Quarantine,2010(5). |
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| Authors: | Xu Lang Yu Daojian Li Jianguang Wang Yinzhu Chen Zhilin |
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| Institution: | Xu Lang1,Yu Daojian 1,Li Jianguang2,Wang Yinzhu3,Chen Zhilin1(1.Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518001,China,2.Beijing Entry-Exit Inspection and Quarantine Bureau,3.Institute of Entomology and State Key Laboratory for Biocontrol,Sun Yat-Sen University) |
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| Abstract: | Genus Anastrepha Schiner(Diptera:Tephritidae)is a kind of pest for fruits and vegetables with special significance to the world,and also to be as the plant quarantine pest list for China.Because of many species in this genus,It is difficult to identify based on morphological characters.In this study,six sets of specific PCR primers and six probes were obtained based on mtDNA COI gene.Six of important Anastrepha species,Anastrepha fraerculus,A.ludens,A.obliqua,A.distincta,A.striata and A.chiclayae,were rapid... |
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| Keywords: | Anastrepha spp AllGlo fluorescence real-time PCR identification |
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