| 启动子Prd29A的克隆及在百脉根中的活性鉴定 |
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| 引用本文： | 韦正乙,刘艳芝,于志晶,蔡勤安,林春晶,邢少辰.启动子Prd29A的克隆及在百脉根中的活性鉴定[J].吉林农业大学学报,2008,30(5). |
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| 作者姓名： | 韦正乙 刘艳芝 于志晶 蔡勤安 林春晶 邢少辰 |
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| 作者单位： | 吉林省农业科学院生物技术研究中心,长春,130033 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 利用PCR方法,从拟南芥中克隆了865bp的逆境诱导型启动子Prd29A.利用农杆菌介导的瞬时表达方法,结合GUS染色,在豆科植物百脉根的幼苗中进行活性鉴定.结果表明:启动子Prd29A在正常情况下不表现活性,而在逆境(高渗、高盐)和ABA诱导下活性很高,这为该启动子在百脉根等豆科植物中的应用提供了依据.
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| 关 键 词： | 逆境 rd29A启动子 百脉根 |
Cloning of Prd29A and Its Activity Evaluation in Lotus cirniculatus |
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| WEI Zheng-yi,LIU Yan-zhi,YU Zhi-jing,CAI Qin-an,LIN Chun-jing,XING Shao-chen.Cloning of Prd29A and Its Activity Evaluation in Lotus cirniculatus[J].Journal of Jilin Agricultural University,2008,30(5). |
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| Authors: | WEI Zheng-yi LIU Yan-zhi YU Zhi-jing CAI Qin-an LIN Chun-jing XING Shao-chen |
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| Institution: | Biotechnology Research Centre;Jilin Academy of Agricultural Science;Changchun 130033;China |
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| Abstract: | Prd29A,A 865 bp in length of stress induced promoter,was cloned by PCR from A.thaliana.The activity of the promoter was evaluated in the leguminous plant Lotus cirniculatus L.by transient expression via agrobacterium-mediated method united with GUS assay in young seedlings.The results indicated that the promoter Prd29A performed no activity under normal condition,but high activity induced by stresses(high salinity,osmotic) and ABA.These results could facilitate the improvement of leguminous plants transform... |
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| Keywords: | stress rd29A promoter Lotus cirniculatus |
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