| 猪精液提取蓝耳病病毒核酸的方法比较 |
| |
| 引用本文： | 李巍,赵景利,孙华武,苍真伟,王承功,陈明非,高佰华.猪精液提取蓝耳病病毒核酸的方法比较[J].现代畜牧兽医,2019(5):41-44. |
| |
| 作者姓名： | 李巍 赵景利 孙华武 苍真伟 王承功 陈明非 高佰华 |
| |
| 作者单位： | 大连市现代农业生产发展服务中心;辽宁省大连市长海县动物卫生监督所;辽宁春兴畜牧兽医服务中心 |
| |
| 摘 要： | 为筛选从猪精液中提取微量蓝耳病病毒(PRRSV)核酸的有效方法,将蓝耳病(变异株)弱毒苗稀释成不同滴度(TCID50/mL)的病毒液,与健康猪常温精液混合作用。分别用Trizol法、磁珠法、离心柱法提取含毒精液中的病毒RNA,用荧光RT-PCR试验检测病毒RNA,结果表明,当精液中病毒滴度高于0.1 TCID50/mL时,3种病毒RNA提取方法均可检出。离心柱法对猪精液中PRRSV核酸提取灵敏度最高,可检出0.01 TCID50/mL滴度的病毒,稳定性好。
|
| 关 键 词： | 蓝耳病 猪精液 含毒精液 病毒RNA 提取 |
Comparison of Extraction Methods for PRRSV RNA from Boar Semen |
| |
| Li Wei,Zhao Jingli,Sun Huawu,Cang Zhenwei,Wang Chenggong,Chen Mingfei,Gao Baihua.Comparison of Extraction Methods for PRRSV RNA from Boar Semen[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2019(5):41-44. |
| |
| Authors: | Li Wei Zhao Jingli Sun Huawu Cang Zhenwei Wang Chenggong Chen Mingfei Gao Baihua |
| |
| Institution: | (Dalian Production and Development Service Center for Modern Agriculture,Liaoning Dalian 116037;Dalian Center for Animal Disease Prevention and Control,Liaoning Dalian 116037;Liaoning Chunxing Animal Husbandry and Veterinary Service Center,Liaoning Shenyang 110001) |
| |
| Abstract: | Li Wei;Zhao Jingli;Sun Huawu;Cang Zhenwei;Wang Chenggong;Chen Mingfei;Gao Baihua(Dalian Production and Development Service Center for Modern Agriculture,Liaoning Dalian 116037;Dalian Center for Animal Disease Prevention and Control,Liaoning Dalian 116037;Liaoning Chunxing Animal Husbandry and Veterinary Service Center,Liaoning Shenyang 110001) |
| |
| Keywords: | Porcine reproductive and respiratory syndrome Boar semen Semen containing PRRSV Virus RNA Extract |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
