小孢子培养有效获得松花型花椰菜DH再生植株

对5个松花型花椰菜杂种一代的小孢子培养研究表明，小孢子胎胚发生主要依赖于基因型，庆农65天的每花蕾胚状体产量最高，平均达15.5个。松花菜的胚状体萌发率一般在30%左右，并有效获得了大量的DH再生植株。冷击预处理能显著影响花椰菜小孢子的胚胎发生，但供体材料间存在不同的结果。结球期和开花结角期再生植株的生育期与育性出现较大分离，可育且能正常结角的比例约占全部小孢子再生植株的50%以上，因而不再需要加倍处理。

Efficient Production of Doubled Haploid Plants from Microspore Culture in Loose-Curd Cauliflowers (Brassica oleracea var. botrytis L.)

Five loose-curd-type cauliflower hybrids were applied to induce embryogenesis and plant regeneration via isolated microspore culture. The results indicated that the embryogenesis was mainly genotype-dependency. The highest embryo production was found in Chinglong 65, which produced an average yield of 15.5 embryos per flower bud. The germinated rate for microspore-derived embryos in loose curd cauliflowers was normally at 30%, meanwhile a great number of doubled haploid plants were obtained. Cold pretreatment to flower buds had a significant influence upon microspore embryogenesis, and a contradictory result was given regarding to tested genotypes. A big variation in growth stage and fertility for regenerated plants at heading stage and anthesis was observed, showing above 50% plants giving normal seed set and fertile capacity, which implies no longer doubling treatment in cauliflower microspore culture.