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| 供体细胞和曲古抑菌素(TSA)对奶牛克隆胚胎发育的影响 | |
| 引用本文： | 章美玲,张运海,陶勇,刘亚,曹鸿国,李运生,Oto Hanus,Josef Kucera,章孝荣.供体细胞和曲古抑菌素(TSA)对奶牛克隆胚胎发育的影响[J].农业生物技术学报,2011,19(4). |
| 作者姓名： | 章美玲 张运海 陶勇 刘亚 曹鸿国 李运生 Oto Hanus Josef Kucera 章孝荣 |
| 作者单位： | 1. 安徽农业大学动物科技学院,合肥230036;合肥市妇幼保健院,合肥230001 2. 安徽农业大学动物科技学院,合肥,230036 3. Research Institute for Cattle Breeding, Rapotin,The Czech Republic |
| 基金项目： | 国家高技术研究发展计划(863)重点项目(No.2008AA101003); 安徽省十一五重大科技专项(No.08010301066); 安徽省国际科技合作计划项目(No.08080703034); 安徽省科技攻关项目(No.08010301066)共同资助 |
| 摘 要： | 本研究探讨供核细胞处理策略(新鲜消化、-196℃冻存组(复苏后)和曲古抑菌素(TSA)处理组)和TSA-CR1aa胚胎培养液处理时间对克隆胚发育的影响。利用体细胞核移植技术生产奶牛克隆胚胎,将部分克隆胚移植给自然发情同步的受体,检验克隆胚的体内发育能力。结果显示:TSA处理体细胞组的克隆胚卵裂率和囊胚率与新鲜消化和-196℃冻存组相比差异显著(P<0.05);-196℃冻存组与新鲜消化组差异不显著;用TSA-CR1aa分别处理新鲜消化细胞构建的克隆胚0、24、48和60h,其中,60h处理组的囊胚率最高(36.11±1.78%)与其他组对比差异显著(P<0.05);用TSA-CR1aa分别处理TSA处理组构建的重构胚24、48和60h,结果发现,60hTSA-CR1aa处理组囊胚率(37.39±1.78%)最高,显著高于24h(25.48±1.34%)TSA-CR1aa处理组,且差异显著(P<0.05)。将所获得的克隆胚胎分别移植给40头自然发情的受体母牛,并观察移植25,45,65和90d后的返情情况,结果显示,各组受孕率无统计学差异,但经TSA处理细胞和胚胎组移植的受体中,获得一头存活的体细胞克隆奶牛。说... |
| 关 键 词： | 曲古抑菌素(TSA) 体细胞核移植 奶牛 |
Effect of Donor Cell and Trichostatin A(TSA) on Development of Cloned Dairy Cattle Embryos |
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| Zhang Meiling,Zhang Yunhai,Tao Yong,Liu Ya,Cao Hongguo,Li Yunsheng,Oto Hanus,Josef Kucera,Zhang Xiaorong.Effect of Donor Cell and Trichostatin A(TSA) on Development of Cloned Dairy Cattle Embryos[J].Journal of Agricultural Biotechnology,2011,19(4). | |
| Authors: | Zhang Meiling Zhang Yunhai Tao Yong Liu Ya Cao Hongguo Li Yunsheng Oto Hanus Josef Kucera Zhang Xiaorong |
| Institution: | Zhang Meiling1,2 Zhang Yunhai1* Tao Yong1 Liu Ya1 Cao Hongguo1 Li Yunsheng1 Oto Hanus3 Josef Kucera3 Zhang Xiaorong1* 1 College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China,2 Hefei Maternity and Child Health Hospital,Hefei 230001,3 Research Institute for Cattle Breeding,Rapotin,The Czech Republic |
| Abstract: | The objective of present study was to investigate the effects of treatments to donor cells with fresh digestion(FD),cryopreservation/thawning(CT),trichostatin A(TSA) and durations of culture using TSA-CR1aa medium on in vitro development of dairy cow cloned embryos.In addition,some somatic cell cloned embryos were transferred to surrogates in heat to evaluate the in vivo developmental competence.The results showed that pretreatment of donor cells using TSA could significantly increase both cleavage and blas... |
| Keywords: | Trichostatin A Somatic cell nuclear transfer Dairy cow |
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