| 山西省马铃薯Y病毒pl基因的克隆与序列分析 |
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| 引用本文： | 吴兴泉,刘晓磊,陈士华,曹成,陈琼珍,侯志鹏.山西省马铃薯Y病毒pl基因的克隆与序列分析[J].河南农业科学,2009(5). |
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| 作者姓名： | 吴兴泉 刘晓磊 陈士华 曹成 陈琼珍 侯志鹏 |
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| 作者单位： | 河南省谷物资源转化与利用省级重点实验室,河南工业大学,河南,郑州,450052 |
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| 基金项目： | 河南省教育厅自然科学基础研究项目，河南工业大学校科研基金项目 |
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| 摘 要： | 依据马铃薯Y病毒基因组序列,设计合成了1对引物,以从山西省获得的带毒马铃薯叶片总RNA为模板,通过RT-PCR扩增得到852 bp的基因片段并进行了序列测定。BLAST分析表明,该DNA序列与PVY N:O株系pl基因序列相似性最高可达99%。
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| 关 键 词： | 山西省 马铃薯Y病毒 pl基因 |
Cloning and Sequence Analysis of Potato Virus Y pl Gene from Shanxi Province |
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| WU Xing-quan,LIU Xiao-lei,CHEN Shi-hua,CAO Cheng,CHEN Qiong-zhen,HOU Zhi-peng.Cloning and Sequence Analysis of Potato Virus Y pl Gene from Shanxi Province[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | WU Xing-quan LIU Xiao-lei CHEN Shi-hua CAO Cheng CHEN Qiong-zhen HOU Zhi-peng |
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| Institution: | The Key Lab of Conversion and Utilization of Grain Resource of Henan Province;Henan University of Technology;Zhengzhou 450052;China |
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| Abstract: | A pair of specific primers was designed based on the gene sequence of potato virus Y,which amplified a 852 bp DNA fragment from the total RNA of potato leaves infected by PVY collected from Shanxi province by RT-PCR.Sequence analysis showed that the DNA fragment amplified had 99% similarity with that of pl gene of PVY strain N:O. |
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| Keywords: | Shanxi province Potato virus Y pl gene |
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