| 马铃薯卷叶病毒的鉴定与提纯 |
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| 引用本文： | 王人元,吕文清.马铃薯卷叶病毒的鉴定与提纯[J].中国马铃薯,1988(4). |
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| 作者姓名： | 王人元 吕文清 |
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| 作者单位： | 东北农学院，东北农学院 |
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| 摘 要： | 自东北农学院育种试验地的马铃薯植株上所分离的14个具有卷叶症状的病毒标样经生物鉴定、蚜传特性、粒体形态和血清反应等鉴定均为PLRV。分离物85-1在曼陀罗上繁殖,经PEG沉淀,两次差速离心和蔗糖梯度离心后的得毒率为0.1mg/kg材料。提纯材料以接种后4～6周的曼陀罗最好。
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IDENTIFICATION AND PURIFICATION OF POTATO LEAFROLL VIRUS |
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| Abstract: | All 14 virus samples bearing leafroll symptom isolated from potato plants in potato breeding experimental fields in Northeast Agricultural College belong to PLRV according to bioassays,particle shape,transmission character by aiphids and serological reaction.The isolate 85-1 was propagated on Datura stramonium and purified by PEG precipitation,two cycles of differential centri- fugation and sucrose density gradient centrifugation.The virus yield was 0.1 mg/kg of fresh plant material.In our experiments the best material for purification was Datura stramonium inoculated 4-6 weeks before purification. |
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