兔多杀性巴氏杆菌的分离鉴定及耐药性研究

从病死兔肝脏中分离获得1株两极着色、革兰氏阴性球杆菌,根据发病情况、病理变化、菌株形态特征、培养特性、生理生化特性及其16S rRNA序列测定结果,确定为兔多杀性巴氏杆菌。通过动物试验证实,该分离菌株具有较强的致病性;药敏特性检测结果表明,该分离菌株对常用抗生素有较强的耐药性,在所检测的22种抗生素中仅对氟苯尼考、头孢氨苄、磺胺六甲氧嘧啶、头孢噻肟、洛美沙星、菌必治等8种抗生素高度敏感,因此防治时应进行药敏试验筛选敏感药物;耐药基因检测发现,该分离菌株携带有多种耐药基因,与药敏试验结果一致。

Isolation and identification of rabbit's Pasteurella multocida and its antimicrobial resistance

A strain of gram-negative coccobacillus,with darkened ends,was isolated from a rabbit.Based on pathological changes,morphological,cultural,physiological and biochemical characteristics as well as the sequencing results of 16S rRNA,it was identified as rabbit Pasteurella multocida.The laboratory tests proved that this isolated strain had strong pathogenicity.Drug sensitivity characteristics showed that it had strong resistance to commonly used clinical antibiotics.Out of 22 antibiotics tested on the strain,it showed high sensitivity to florfenicol,cephalexin,sulfamonomethoxine,cefotaxime,lomefloxacin and ceftriaxone,while was immune to others.As such,the drug sensitivity tests should be undertaken to select the drug for controlling the rabbit Pasteurella multocida.