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The use of microsatellite polymorphisms for the identification of Australian breeding lines of rice (Oryza sativa L.)
Authors:SH Garland  L Lewin  M Abedinia  R Henry  A Blakeney
Institution:(1) Centre for Plant Conservation Genetics, Southern Cross University (SCU), Lismore, NSW, 2480, Australia;(2) Yanco Agricultural Institute, NSW Agriculture, Yanco, NSW, 2703, Australia;(3) Grains Processing, BRI Australia Ltd, North Ryde, NSW, 2113, Australia
Abstract:Ten microsatellite loci were analysed for 43 cultivars or breeding lines of rice. Polymorphism-Information-Content values ranged from 0.62 to 0.92. The microsatellite markers were found to be useful for cultivar identification and assessment of genetic relationships. Most of the cultivars could be uniquely identified by at least one microsatellite marker. Genetic heterogeneity was detected within rice samples by amplification of microsatellites from DNA extracted from multiple individual plants and also from bulked DNA preparations. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:microsatellite  Oryza sativa  polymorphism  rice
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