活性炭对小白菜游离小孢子培养的影响

以24个小白菜品种为材料,通过在NLN培养基中添加不同浓度活性炭,研究其对游离小孢子培养的影响.结果表明:在NLN-13培养基中添加活性炭(0.5 g/L),有利于小孢子胚的诱导和形成,供试材料(N4,N8)添加活性炭处理胚诱导率较未添加活性炭的处理分别提高19,66.5倍.采用添加活性炭(0.5 g/L)的NLN培养基对24份小白菜品种进行游离小孢子培养,有20份材料诱导出胚,培养成功率为83.3%.表明添加活性炭的培养基对小白菜游离小孢子培养有较好的效果.不同基因型间胚诱导率差别较大,每花蕾诱导胚数为0.4～86.7个.依据胚诱导率的高低可将其分为极易诱导、易诱导、难诱导和不能诱导出胚4类.B5 6-BA 0.2 mg/L NAA 0.02 mg/L 活性炭0.5 g/L 3%蔗糖 1%琼脂培养基有利于幼胚长成植株.

Influence of Activated Charcoal on Isolated Microspore Culture in Chinese Cabbage

Fluency of activated charcoal on isolated microspore culture in Chinese cabbage was studied.The results showed that NLN-13 liquid medium with activated charcoal(0.5 g/L)was obviously better in frequency of microspore-derived embryos and frequency of regenerated plants.Frequency of microspore-derived embryos in N4 and N8 was increased 19 and 66.5 times.Isolated microspores from 24 varieties of Chinese cabbage were cultured in NLN-13 liquid medium with activated charcoal(0.5 g/L),20 varieties were produced mi...