EST‐SSR analysis provides insights about genetic relatedness,population structure and gene flow in grass pea (Lathyrus sativus) |
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Authors: | Khela R. Soren Ashutosh Yadav Gaurav Pandey Priyanka Gangwar Ashok K. Parihar Abhishek Bohra Girish P. Dixit Subhojit Datta Narendra Pratap Singh |
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Affiliation: | 1. Division of plant Biotechnology Indian Institute of Pulses Research, Kanpur, India;2. Division of Genetics, Indian Agricultural Research Institute, New Delhi, India;3. Department of Biotechnology, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, India;4. Crop Improvement Division, Indian Institute of Pulses Research, Kanpur, India;5. MuLLaRP Unit, Indian Institute of Pulses Research, Kanpur, India |
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Abstract: | Grass pea (Lathyrus sativus) is an important food‐legume crop for resource‐poor farmers in the developing world. However, given its cultivation in the most underprivileged regions, the crop has not received appropriate scientific attention particularly from the genomic perspective, thereby giving it a status of genomic orphan. Nevertheless, some recent studies have attempted to develop modern molecular tools to strengthen the genetic and genomic research. In the present investigation, a comprehensive collection comprising 176 accessions was analysed using EST‐simple sequence repeats (SSRs). The SSR analysis revealed existence of a total of 51 alleles with an average polymorphic information content value of 0.35. A moderate level of gene diversity was noticed that ranged from 0.04 to 0.73 with an average of 0.43. Noticeably, two distinct subpopulations were recovered using cluster analysis. In addition, the presence of admixtures in population reflected the strong possibilities of gene flow between the accessions across the geographical boundary. In summary, we provide additional insights about the informativeness of available EST‐SSR markers along with an extended understanding of relatedness, genetic structure and gene flow in an under‐researched legume crop. |
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Keywords: | genetic diversity grass pea molecular variance population structure principal component analysis simple sequence repeat |
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