红掌组培苗生根培养基的优化

目的]优化红掌组培苗的生根技术体系。方法]以红掌愈伤组织诱导获得的无菌试管苗为外植体,采用4因素4水平L16(45)正交试验,探讨NAA、IBA、蔗糖和活性炭等因素对红掌组培苗生根效果的影响。结果]在MS基本培养基添加IBA 0.5 mg/L、活性炭0.7 g/L和蔗糖30 g/L(即A1B4C4D4组合)条件下,诱导生根效果较好;生长素IBA诱导生根的效果优于NAA;极值分析表明影响红掌组培苗生根的因素为IBA活性炭NAA蔗糖,4因素对红掌生根均有影响,但未达到显著差异。结论]红掌生根培养基的最优组合为A3B4C3D4,即添加NAA 0.2 mg/L、IBA 0.5 mg/L、活性炭0.5 g/L和蔗糖30 g/L的MS培养基对红掌组培苗生根最为有利。

Optimization of Rooting Medium of the Plantlets in Anthurium andraeanum

The research aimed to optimize the rooting technology system of the plantlets in Anthurium andraeaum. Method]Plantlets inducted from Anthurium callus were used as explants,and the influence of auxin NAA and IBA,sucrose and activated charcoal were investigated through the method of orthogonal test L16（45）. Result]The results showed that the suitable medium for inducing roots was MS medium with IBA 0. 5 mg /L,activated charcoal 0. 7 g /L and sugar 30 g /L,and the induce effect of auxin IBA was better than that of NAA. Based on the Extremum analysis results,four factors all affected the rooting,and their effects on plantlet rooting was IBA active charcoal NAA sucrose,though didn＇t reach a significant difference levels. Conclusion] The optimal combination for Anthurium plantlets rooting medium was A3B4C3D4,which is MS medium with 0.2 mg/L NAA,0.5 mg/L IBA,0.5 g/L activated charcoal and 30 g/L sugar.