| 一种棉花细胞质雄性不育系线粒体RNA的提取方法 |
| |
| 引用本文： | 巩养仓,张兴平,邢朝柱,王洪,吴建勇,郭立平,彭凡嘉.一种棉花细胞质雄性不育系线粒体RNA的提取方法[J].安徽农业科学,2014(29):10097-10098. |
| |
| 作者姓名： | 巩养仓 张兴平 邢朝柱 王洪 吴建勇 郭立平 彭凡嘉 |
| |
| 作者单位： | 1. 湖南省棉花科学研究所,湖南常德,415101
2. 中国农业科学院棉花研究所,河南安阳,455000 |
| |
| 摘 要： | 目的]获得高质量的棉花细胞质雄性不育系线粒体RNA。方法]以哈克尼西棉细胞质雄性不育系黄化苗为研究对象,对棉花线粒体RNA的提取方法进行探讨。结果]采取先分离出棉花线粒体再提取RNA的方法,最终获得了质量较好的线粒体RNA(mtRNA)。结论]成功获得了质量较高的mtRNA。
|
| 关 键 词： | 棉花 线粒体RNA提取 方法 |
A Method for Mitochondrial RNA Isolation of Cytoplasmic Male Sterility in Cotton |
| |
| Institution: | GONG Yang-cang,ZHANG Xing-ping,XING Chao-zhu. et al (Hunan Cotton Research Institute, Changde, Hunan 455000; Cotton Re- search Institute, CAAS, Anyang,Hcnan 455000) |
| |
| Abstract: | The aim was to obtain high quality mitochondrial RNA isolation of cytoplasmic male sterility in cotton. Method]Taking CMS line of G. harknessii as material,a method of mitochondrial RNA isolation was constructed. Result]The protocol was that cotton mitochondron was isolated firstly and then mtRNA was extracted using an improved CTAB method. And finally the higher quality mtRNA was obtained.Conclusion]The study successfully obtain higher quality mtRNA. |
| |
| Keywords: | Cotton mtRNA extraction Method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
