| 阳离子树脂纯化大豆皂苷研究 |
| |
| 引用本文： | 王东冬,师文添,赵大云. 阳离子树脂纯化大豆皂苷研究[J]. 上海交通大学学报(农业科学版), 2007, 25(4): 356-362 |
| |
| 作者姓名： | 王东冬 师文添 赵大云 |
| |
| 作者单位： | 上海交通大学,食品科学与工程系,上海,201101;上海交通大学,食品科学与工程系,上海,201101;上海交通大学,食品科学与工程系,上海,201101 |
| |
| 摘 要： | 以大孔吸附树脂提取的大豆皂苷粗提物为研究对象,利用阳离子交换树脂进行纯化。以HPLC分析方法,苷元显色法等多种分析手段,检测树脂纯化的除杂和脱色能力,验证此方法的可行性。通过实验研究,确定了最佳纯化条件:选用50%甲醇水溶液,最佳pH为6.0。本法除杂率达33.01%,脱色率达52.36%。此法不仅有效地去除了样品中的色素等杂质,还可以去除部分大豆异黄酮类物质。
|
| 关 键 词： | 大豆皂苷 阳离子交换树脂 纯化 |
| 文章编号： | 1671-9964（2007）04-0356-07 |
| 收稿时间： | 2007-01-30 |
| 修稿时间： | 2007-01-30 |
Research on Cation Exchange Resin Applied in Soyasaponin Purification |
| |
| WANG Dong-dong,SHI Wen-tian,ZHAO Da-yun. Research on Cation Exchange Resin Applied in Soyasaponin Purification[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2007, 25(4): 356-362 |
| |
| Authors: | WANG Dong-dong SHI Wen-tian ZHAO Da-yun |
| |
| Affiliation: | Department of Food Science and Technology, Shanghai Jiaotong University, Shanghai 201101, China |
| |
| Abstract: | A cation exchange resin was applied to purify the crude soyasaponins obtained by solid phase extraction(SPE) using AB-8,a kind of polystyrene matrix macroporous absorbent resins.The purification and decoloration effects were evaluated by means of HPLC and colorimetric determinations.The results revealed that the optimized conditions of the purification procedure as following: 50% methanol aqueous solution with pH=6.0.The results also showed that the method could remove contained impurity substances as high as 33.01% as well as of 52.36% decoloration,the removed impurities are colorants and part of isoflavonoids in dominant. |
| |
| Keywords: | soyasaponin cation exchange resin purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《上海交通大学学报(农业科学版)》浏览原始摘要信息 |
|
点击此处可从《上海交通大学学报(农业科学版)》下载全文 |
|
