| 玻璃化冻存对羊软骨细胞的影响 |
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| 引用本文： | 黄佩龙,张绍志,虞效益,胡军祥. 玻璃化冻存对羊软骨细胞的影响[J]. 浙江林学院学报, 2011, 28(2): 269-274 |
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| 作者姓名： | 黄佩龙 张绍志 虞效益 胡军祥 |
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| 作者单位： | 1. 浙江农林大学,林业与生物技术学院,浙江,临安,311300
2. 浙江大学,制冷与低温研究所,浙江,杭州,310027 |
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| 基金项目： | 国家自然科学基金资助项目(50606032); 浙江省自然科学基金资助项目(Y304053) |
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| 摘 要： | 对玻璃化冻存后羊Ovis aries软骨细胞的存活率和琥珀酸脱氢酶（SDH）活性进行了初步研究。结果表明：细胞存活率随着冻存时间的延长而逐渐下降，但下降速度不明显；SDH活性在玻璃化冻存1 ~ 20 d变化幅度较大，20 ~ 30 d下降趋势减弱，保持相对稳定。从各项指标来看，认为玻璃化溶液VSb组的冻存效果最好，在经过5，10， 15，20和30 d的冻存后，其存活率分别为85.47% ± 1.78%，80.73% ± 1.81%，78.62% ± 2.06%, 76.35% ± 2.58%和73.83% ± 1.49%，细胞SDH活性吸光度值分别为0.49 ± 0.064，0.444 ± 0.073，0.394 ± 0.039，0.354 ± 0.082和0.339 ± 0.053。该玻璃化冻存方法对长期冻存羊软骨细胞具有一定的实际应用意义。图4表2参17
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| 关 键 词： | 动物学 玻璃化冻存 软骨细胞 存活率 琥珀酸脱氢酶 绵羊 |
Vitrification of chondrocyte from Ovis aries |
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| HUANG Pei-long,ZHANG Shao-zhi,YU Xiao-yi,HU Jun-xiang. Vitrification of chondrocyte from Ovis aries[J]. Journal of Zhejiang Forestry College, 2011, 28(2): 269-274 |
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| Authors: | HUANG Pei-long ZHANG Shao-zhi YU Xiao-yi HU Jun-xiang |
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| Affiliation: | HUANG Pei-long1,ZHANG Shao-zhi2,YU Xiao-yi2,HU Jun-xiang1(1.School of Forestry and Biotechnology,Zhejiang A & F University,Lin'an 311300,Zhejiang,China,2.Institute of Refrigeration and Cryogenics,Zhejiang University,Hangzhou 310027,China) |
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| Abstract: | The effects of vitrification on survival rate and succinate dehydrogenase（SDH） activity of ovine chondrocytes were studied.Results showed that the SDH activity decreased remarkably within 20 d after vitrification,whereas the survival rate decreased slowly.VSb selected according to the trend of survival rates and vitality of SDH.After being cryopreserved for 5,10,15,20 and 30 days,the survival rates were 85.47% ± 1.78%,80.73% ± 1.81%,78.62% ± 2.06%,76.35% ± 2.58% and 73.83% ± 1.49%,and the vitality of SDH showed 0.49 ± 0.064,0.444 ± 0.073,0.394 ± 0.039,0.354 ± 0.082 and 0.339 ± 0.053.This indicated that cryopreservation of ovine chondrocytes by vitrification had some practical value. |
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| Keywords: | zoology vitrification chondrocytes survival rate succinate dehydrogenase(SDH) Ovis aries |
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