| 不同蛋白酶水解模拟餐厨垃圾优化工艺研究 |
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| 引用本文： | 朱振新,许士洪,李登新.不同蛋白酶水解模拟餐厨垃圾优化工艺研究[J].安徽农业科学,2012,40(35):17267-17269. |
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| 作者姓名： | 朱振新 许士洪 李登新 |
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| 作者单位： | 东华大学环境科学与工程学院,上海,201620 |
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| 基金项目： | 上海市自然科学基金项目，中央高校基本科研业务费专项，上海市重点学科建设项目 |
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| 摘 要： | 目的]研究不同蛋白酶水解模拟餐厨垃圾的优化工艺。方法]通过单因素试验,用牛肉模拟餐厨垃圾,研究了木瓜蛋白酶、中性蛋白酶、碱性蛋白酶、胰蛋白酶和胃蛋白酶水解牛肉的优化工艺。结果]较其他几种酶,胰蛋白酶水解效果最好,水解度最大可达到74.18%。胰蛋白酶水解的最适条件:酶用量2%,pH 7.5,固液比为1∶60,水解时间2 h,水解温度50℃。结论]该研究为解决餐厨垃圾的同源性污染问题打下了基础。
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| 关 键 词： | 蛋白酶 水解 餐厨垃圾 优化工艺 |
Study on Optimal Hydrolyzation Condition of the Simulated Food Waste with Different Proteases |
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| Institution: | ZHU Zhen-xin et al(College of Environmental Science and Engineering,Donghua University,Shanghai 201620) |
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| Abstract: | Objective] The research aimed to study optimal hydrolyzation condition of the simulated food waste with different proteases.Method] Via single-factor test,beef as the simulated food waste,the optimal technics of papain,neutrase,alcalase,trypsin and pepsin hydrolyzing beef were studied.Result] Compared with other proteases,the maximum hydrolysis degree for trypsin was 74.18%,and its hydrolysis effect was the best.The optimal hydrolysis condition of trypsin was 2% of enzyme dosage,pH 7.5,1∶60 of solid-liquid ratio,2 h of hydrolysis time and 50 ℃ of hydrolysis temperature.Conclusion] The research laid foundation for solving homology pollution problem of the food waste. |
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| Keywords: | Protease Hydrolysis Food waste Optimal technic |
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