猪传染性胃肠炎和猪流行性腹泻诊断方法研究进展

猪传染性胃肠炎和猪流行性腹泻均是由冠状病毒引起的以仔猪发生呕吐、腹泻和脱水为特征的两种传染病。目前 ,对这两种传染病的诊断方法主要有病毒中和试验 ,免疫荧光法 ,免疫电镜法 ,EL ISA法和 RT-PCR法。随着分子生物学的不断发展 ,EL ISA法和 RT-PCR法以其敏感性高、特异性强越来越受到人们的重视。文章就各种诊断方法近年来的研究进展进行了综述 ,并简明地指出了研究中存在的问题以及未来展望 ,可为这两种传染病诊断方法的进一步研究提供一些新的思路

Research Progress on Diagnostic Methods to Porcine transmissible gastroenteritis and Porcine epidemic diarrhea

Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are two kinds of highly contagious diseases caused by two members of the Coronaviridae respectively, characterized in piglets primarily by vomiting and watery diarrhoea and dehydration. At present,main diagnostic methods to TGE and PED are virus neutralization(VN),immunofluorescence(IF),immune electron microscopy(IEM),ELISA and RT-PCR. With the development of molecular biology, the diagnostic methods of ELISA and RT-PCR are becoming more important than before for their sensitivity and specialization. This article reviewed the progresses of diagnostic methods to TGE and PED in recent years,we also briefly discussed its problems at present and developments in future,and provided some new ideas for further research on diagnostic methods to the two diseases.