哈密瓜细菌性果斑病菌快速检测方法的建立

 【目的】哈密瓜细菌性果斑病在中国为新发生的病害，其病原菌为燕麦食酸菌西瓜亚种（Acidovorax avenae subsp. Citrulli），危害瓜果造成品质下降。该病为典型的种传细菌性病害，因此种子检疫成为防治此病害的重要手段。【方法】通过实验，将生物学、免疫学和分子生物学检测技术有机结合，建立了一套针对哈密瓜果斑病的种子带菌检测方法Bio-IMS-Real-time PCR。【结果】经过人工模拟种子带菌检测实验，结果表明该方法可成功地检测出1 000粒种子中的1粒带菌种子（带菌量约为1.04×105 CFU/种子，经计算，可以检测的最初浓度为2 CFU?ml-1 ASCM培养液），且信号较强。【结论】该检测方法的建立，大大提高了对哈密瓜细菌性果斑病菌检测的精度，也为生产实践中哈密瓜果斑病的防治提供了重要的技术支持。

Establishment of Rapid Detection Method for the Pathogen of Hami Melon Fruit Blotch

【Objective】Hami melon bacterial fruit blotch (HBFB) is a new disease in China, the pathogen is Acidovorax avenae subsp. citrulli, which damages the fruits and lowers their qualities. HBFB is a typically seedborne bacterial disease, and the important prevention and cure method is the quarantine of the seeds with bacteria.【Method】and【Result】In this study, the immuno-detection was combined with PCR by using the superparamagnetic beads to form IMS-Realtime-PCR, which increase the precision of bacteria detection to app. 3×102 CFU/ml. The integrated method of bacterial detection was formed through combining the ASCM culturing, IMS and real-time PCR. 【Conclusion】One seed with bacteria in 1000 seeds could be detected using this integrated method.