高温胁迫下转甜椒正义甘油-3-磷酸酰基转移酶基因（GPAT）烟草缓解PSⅡ光抑制

 【目的】探讨类囊体膜脂组成与高温光抑制的关系。【方法】以转甜椒正义甘油-3-磷酸酰基转移酶基因（GPAT）的烟草植株为试验材料，测定了类囊体膜脂组成，高温胁迫后的光合参数和叶绿素荧光参数。【结果】转基因烟草植株类囊体膜的单半乳糖甘油二脂（MGDG），双半乳糖甘油二脂（DGDG），硫代异鼠李糖甘油二脂（SQDG）和磷脂酰甘油（PG）的饱和程度均上升，其中MGDG饱和程度增加了16.2%，上升幅度最为显著。随胁迫温度的升高，转基因烟草植株与野生型植株Fv/Fm，ΦPSⅡ和Pn逐渐下降，但转基因植株下降幅度小于野生型，48℃时差异最为显著。【结论】转基因烟草植株类囊体膜脂脂肪酸饱和度增加，提高了PSⅡ热稳定性，缓解了PSⅡ光抑制。

Tobacco Transformed with Sweet Pepper Sense GPAT Alleviates PSⅡ Photoinhibition Under High Temperature Stress

The saturated extent of MGDG, SQDG, DGDG and PG in thylakoid membrane of tobacco plants transformed with sweet pepper positive GPAT gene increased entirely, remarkably in MGDG with increasing by 16.2％. Under high temperature stress (48℃), ΦPSⅡ and Fv/Fm of wild type tobacco plants decreased by 42.3% and 21.3%, but only decreased by 24.2% and 13.7% in transgenic tobacco, which indicated that the transformed tobacco plants maintained higher actual photochemistry efficiency of PSII and lower photoinhibition under high temperature. The increase in saturated extent of thylakoid membrane lipids raised the temperature inducing phase separation and enhanced the stability of PSⅡunder high temperature.