育苗阶段虾夷扇贝幼体中可培养细菌的多样性

为了研究虾夷扇贝Patinopecten yessoensis育苗过程中5个发育时期(受精卵时期S1、担轮幼虫时期S2、D形幼虫时期S3、壳顶幼虫时期S4、稚贝时期S5)幼体所含可培养细菌的多样性,采用2216E平板涂布法,从每个发育时期的幼体样品中各分离出30株细菌,并对150株菌进行16S rRNA基因测序分析。结果表明:150株细菌归属于2门3纲8目12科16属39种;在门水平,5个时期幼体样品中变形菌门均为优势门,其次为厚壁菌门;在属水平,优势属为弧菌属(51/150)、假交替单胞菌属(43/150)、芽孢杆菌属(16/150)、交替单胞菌属(9/150),占总数的79.3%;其中弧菌属在S1(43.3%)、S2(36.7%)、S3(36.7%)时期均为优势属,在S1时期,次优势属依次为假交替单胞菌属(26.7%)、动性球菌属(10.0%)和枝芽孢杆菌属(6.7%),在S2时期,次优势属依次为假交替单胞菌属(23.3%)、亚硫酸杆菌属(20.0%)和芽孢杆菌属(13.3%),在S3时期,次优势属依次为假交替单胞菌(16.7%)、动性球菌属(13.3%)和交替单胞菌属(10.0%);假交替单胞菌在S4(36.7%)和S5(40.0%)时期均为优势属,在S4时期,次优势属依次为弧菌属(30.0%)、芽孢杆菌属(16.7%)和交替单胞菌属(10.0%),在S5时期,次优势属依次为弧菌属(23.3%)、芽孢杆菌属(16.7%)和Pontibacillus(10.0%)。研究表明,虾夷扇贝育苗过程中不同发育时期的幼体所含可培养细菌种类比较丰富,细菌群落结构存在较大差异。

Culturable bacterial diversity in larval breeding in Yesso scallop Patinopecten yessoensis

The present study was aimed to examine the cultivable bacterial community associated with Yesso scallop Patinopecten yessoensis larval development stages(fertilized egg S1, trochophora S2, D-shaped larva S3,umbo larva S4 , and juvenile scallop S5 ) . Thirty bacterial strains were isolated from samples in each larval stage by Zobell 2216E, and 150 bacterial strains were identified by polymerase chain reaction and partial 16S rRNA gene sequen-cing. Results showed that all isolates were found to be in 2 phyla,3 classes,8 orders,12 families, 16 genera and 39 species,with the most abundant Proteobacteria, followed by Firmicutes across the five developmental stages. At ge-nus level, the dominant genera were Vibrio (51/150), Pseudoalteromonas (43/150), Bacillus (16/150)and Al-teromonas (9/150), accounting for 79. 3% of the total. Vibrio was dominant genus in stages of S1(43. 3%), S2 (36. 7%), S3 (36. 7%), followed by Pseudoalteromonas (26. 7%), Planococcus (10. 0%) and Virgibacillus (6. 7%)in stage S1, Pseudoalteromonas (23. 3%), Sulfitobacter(20. 0%)and Bacillus(13. 3%)in stage S2, and Pseudoalteromonas (16. 7%), Planococcus (13. 3%) and Alteromonas (10. 0%) in stage S3. However, Pseud-oalteromonas became the dominant in stages of S4(36. 7%)and S5(40. 0%), followed by Vibrio (30. 0%), Ba-cillus(16. 7%)and Alteromonas (10. 0%)in stage S4, and Vibrio (23. 3%), Bacillus (16. 7%)and Pontibacillus ( 10 . 0%) in stage S5 . The findings showed that there were abundant bacterial diversities and different communities among various larval development stages.