| 我国棉花枯萎菌生理小种特异性DNA扩增片段的筛选及序列测定 |
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| 引用本文： | 冯洁,孙文姬,石磊岩. 我国棉花枯萎菌生理小种特异性DNA扩增片段的筛选及序列测定[J]. 植物病理学报, 2000, 30(1): 35-41 |
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| 作者姓名： | 冯洁 孙文姬 石磊岩 |
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| 作者单位： | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100094 |
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| 摘 要： | 利用RAPD技术,以随机引物对我国棉花枯萎菌的3个生理小种(3、7、8号小种)共26个菌株进行PCR扩增,从产生的140个RAPD分子标记中寻找到了不同小种的特征性条带,O PF-10513(3号小种)、OPF-08371(7号小种)及OPF-12703(8号小种)。将其纯化后克隆到pGEM-TEasy质粒载体上,并获得了DNA特异性片段的核酸序列。
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| 关 键 词： | 棉花 枯萎菌 特异性DNA片段 序列测定 |
| 文章编号： | 0412-0914(2000)01-0035-07 |
| 修稿时间： | 1998-10-30 |
SCREENING AND SEQUENCINGOF RACE SPECIAL DNA FRAGMENTS OF Fusarium oxysporum f.sp.vasinfectum IN CHINA |
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| FENG Jie,SUN Wen ji,SHI Lei yan,MA Cun. SCREENING AND SEQUENCINGOF RACE SPECIAL DNA FRAGMENTS OF Fusarium oxysporum f.sp.vasinfectum IN CHINA[J]. Acta Phytopathologica Sinica, 2000, 30(1): 35-41 |
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| Authors: | FENG Jie SUN Wen ji SHI Lei yan MA Cun |
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| Affiliation: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | Random amplified polymorphic DNA(RAPD) technique was used to PCR amplification of 26 strains of races 3,7 and 8 of Fusarium oxysporum f.sp. vasinfectum . The race special DNA fragments OPF 10 513 (race 3),OPF 08 371 (race 7) and OPF 12 703 (race 8) were obtained from 140 RAPD markers, which have been cloned to pGEM T Easy plasmid, and sequences of those DNA fragments have also been achieved. |
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| Keywords: | cotton Fusarium oxysporum f.sp. vasinfectum special DNA fragments sequencing |
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