蒙古栎组织培养的初步研究

以蒙古栎侧芽为外植体对其进行组织培养技术研究,结果表明:在MS+6-BA0.2 mg·L-1+NAA0.01 mg·L-1+蔗糖30 g·L-1的培养基上,外植体分化启动最早;在MS+6-BA0.2 mg·L-1+NAA0.02 mg·L-1+蔗糖30 g·L-1培养基上,芽分化与增殖系数最高,为68.5%,单芽平均高2.87 cm,增殖倍数2.5,芽的高度适中、健壮;在1/2MS+NAA0.05 mg·L-1+蔗糖25 g·L-1培养基上,继代苗生根率最高,生根率达60%。

Study on tissue culture of Quercus mongolica

Lateral buds of Quercus mongolica were taken as explants to study on tissue culture technique .The result showed that the earliest explant differentiation was on the culture medium with MS ＋6-BA0.2 mg· L^-1 ＋NAA0.01 mg· L^-1 ＋saccharose 30 g· L^-1 .The highest budinitiation and growth coefficient was 68.5%on the culture medium with MS ＋6-BA0.2 mg· L^-1 ＋NAA0.02 mg· L^-1 ＋saccharose 30 g· L^-1 , mean height of simple bud was 2.87 cm, proliferation rate was 2.5, the height of the bud is moderate , and the bud was robust .The highest rooting percentage of transgeneration-al seedlings was 60%on the culture medium with 1/2MS＋NAA0.05 mg· L^-1 ＋saccharose25 g· L^-1 .