甘肃省景泰县羊无浆体病的诊断

为建立羊无浆体病简便快捷的病原学检测方法,论文以马米玲等已建立的边缘无浆体MSP5重组抗原间接ELISA检测方法对甘肃省景泰县多地采集的219份田间样品进行羊无浆体ELISA检测,以PCR检测方法进行病原学的检测和验证。同时为进一步验证MSP5基因在边缘无浆体和羊无浆体之间的保守性,Western blot检测证实边缘无浆体重组蛋白在45ku处与羊无浆体阳性血清反应,与羊其他病原阳性血清均不反应,表明该重组蛋白适合作为羊无浆体病的诊断抗原。在被检的219份样品中,ELISA方法检测阳性率为34.7%(76/219),PCR方法阳性率为30.6%(67/219),证实该地区存在羊无浆体病,与以往调查结果相比,阳性率有所下降。利用边缘无浆体MSP5重组抗原建立的EILSA方法具有良好的特异性和敏感性,可以检测羊无浆体病,为羊无浆体病的血清学诊断及流行病学调查提供了手段。

A Diagonsis of Ovine Anaplasmosis in Jingtai Country Gansu Province

In order to establish a convenient and rapid etiology for ovine anaplasmosis ovis detection,a previously established indirect ELISA based on recombinant MSP5 antigen of Anaolasma marginale was applied to investigate 219 sera collected from sheep in Jingtai country,Gansu province.MSP5 is a conserved gene between Anaplasma marginale and Anaplasma ovis.The recombinant MSP5 protein of A.marginale specific reaction with positive serum of A.ovis in 45 ku was observed,and there was no reaction with positive serum samples from other related diseases.PCR detection of the corresponding DNA samples from field samples confirmed the accuracy of the ELISA.In the investigated samples,positive rates of ELISA were 34.7%(76/219) and that of the PCR were 30.6%(67/219).The results confirmed a good specificity and sensitivity of the ELISA and its usefulness for serological detection and epidemiological investigation of ovine anaplasmosis.