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部分禽源生物制品外源病毒的检测
引用本文:庄金秋,梅建国,刘晓倩,王艳,苗立中,沈志强.部分禽源生物制品外源病毒的检测[J].动物医学进展,2012,33(4):121-125.
作者姓名:庄金秋  梅建国  刘晓倩  王艳  苗立中  沈志强
作者单位:1. 山东省滨州畜牧兽医研究院,山东滨州256600;山东绿都生物科技有限公司,山东滨州256600
2. 山东绿都生物科技有限公司,山东滨州,256600
摘    要:进行了部分禽用生物制品外源病毒检测的鸡胚检查法、细胞检查法和鸡检查法。鸡胚检查法应用SPF鸡胚检验;细胞检查法主要通过鸡红细胞吸附试验、禽白血病病毒ELISA和禽网状内皮组织增生症病毒IFA检验疫苗是否含有外源鸡红细胞吸附因子、禽白血病病毒和禽网状内皮组织增生症病毒,并提出疫苗检验的判定标准,为各兽用生物制品生产企业新城疫疫苗的外源病毒检验提供参考。

关 键 词:禽源生物制品  外源病毒  检测

Exogenous Virus Detection of Biological Products of Avian Origin
ZHUANG Jin-qiu , MEI Jian-guo , LIU Xiao-qian , WANG Yan , MIAO Li-zhong , SHEN Zhi-qiang.Exogenous Virus Detection of Biological Products of Avian Origin[J].Progress In Veterinary Medicine,2012,33(4):121-125.
Authors:ZHUANG Jin-qiu  MEI Jian-guo  LIU Xiao-qian  WANG Yan  MIAO Li-zhong  SHEN Zhi-qiang
Institution:1,2(1.Shandong Binzhou Animal Science & Veterinary Medicine Academy,Binzhou,Shandong,256600,China;2.Shandong Lvdu Bio-industry Company,LTD.,Binzhou,Shandong,256600,China)
Abstract:The chicken embryo detection method,cytoscopy method and chicken detection method,which could be used for exogenous virus test of biological products of avian origin were established.Embryo detection method was conducted by using SPF embryoes.Cytoscopy method was used to detect exogenous erythrocyte adsorption factor,avian leukosis virus and avian reticuloendotheliosis virus mainly by chicken erythrocyte adsorption test,avian leukosis virus ELISA detection and avian reticuloendotheliosis virus IFA detection,respectively.The standard of criterion of detection of vaccine was put forword.It could provide reference for exogenous virus detection of biological products of avian origin in veterinary biologics production enterprises.
Keywords:biologic product of avian origin  exogenous virus  detection
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