| 酶法提取鱿鱼皮胶原蛋白工艺条件的研究 |
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| 引用本文： | 郭恒斌,曾庆祝.酶法提取鱿鱼皮胶原蛋白工艺条件的研究[J].南方水产,2008,4(1):58-63. |
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| 作者姓名： | 郭恒斌 曾庆祝 |
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| 作者单位： | 1. 广州陆仕水产企业有限公司,广东,广州,510820
2. 广州大学食品工程系,广东,广州,510006 |
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| 摘 要： | 研究了酶法提取鱿鱼皮胶原蛋白的工艺条件。根据5种蛋白酶水解液中羟脯氨酸（HYP）的含量,确定了胰蛋白酶和木瓜蛋白酶这2种提取率高的酶为水解用酶,采用L16（4^5）正交试验确定了这2种酶水解鱿鱼皮以制备胶原蛋白的最佳酶解条件。结果表明,胰蛋白酶和木瓜蛋白酶水解鱿鱼皮以制备胶原蛋白的最佳温度、加酶量、底物浓度、pH、时间分别为55℃、1200U·g^-1、1：20、pH8.0、4h和50℃、3200U·g^-1、1：20、pH6.0、6h。2种蛋白酶提取的胶原蛋白含量分别为11.08%和11.36%,提取率分别为95.16%和97.56%。
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| 关 键 词： | 鱿鱼皮 胶原蛋白 蛋白酶 水解 |
| 文章编号： | 1673-2227-(2008)01-0058-06 |
| 修稿时间： | 2007年9月10日 |
Study on hydrolytic technics of collagen from squid skin by protease |
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| GUO Hengbin,ZENG Qingzhu.Study on hydrolytic technics of collagen from squid skin by protease[J].South China Fisheries Science,2008,4(1):58-63. |
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| Authors: | GUO Hengbin ZENG Qingzhu |
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| Institution: | GUO Hengbin,ZENG Qingzhu(1. Guangzhou Luxe Seafood Enterprises Ltd.,Guangzhou 510820,China;2. Department of Food Engineering,Guangzhou University,Guangzhou 510006,China) |
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| Abstract: | Hydrolytic technics of collagen from squid skin by protease was studied.Based on the content of hydroxyproline of five kinds of enzymic hydrolysates,trypsin and papain were chosed to apply in the experiment.By L16（4^5） orthogonal experiment design,the optimum processing conditions of extraction by trypsin and papain were determined.Results of extracting collagen show that the optimal conditions of trypsin and papain were temperature 55℃,enzyme dosage 1 200 U·g^-1,concentration of substrate 1：20,pH 8.0,time 4 h,and temperature 50℃,enzyme dosage 3 200 U·g^-1,concentration of substrate 1：20,pH 6.0,time 6 h,respectively.The content of collagen was 11.08% and 11.36%,respectively.Extraction yield of collagen was 95.16% and 97.56%,respectively. |
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| Keywords: | squid skin collagen protease hydrolysis |
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