新型基因工程亚单位菌苗对猪传染性胸膜肺炎的保护效力研究

 利用分泌毒素ApxⅠ、ApxⅡ、ApxⅢ的重组表达产物和胸膜肺炎放线杆菌7型菌，研制了一种新型的基因工程亚单位菌苗并对此疫苗的免疫效力进行了研究。利用该疫苗免疫断奶仔猪，间隔3周加强1次，同时设空白对照组和一种商品化的三价细菌灭活苗组。首免后每周采血检测ApxⅠ、ApxⅡ、ApxⅢ的ELISA抗体和7型菌的血凝抗体，第2次免疫两周后用猪胸膜肺炎放线杆菌1型菌株进行气管攻毒。从攻毒后症状、细菌分离、抗体检测、死后剖检对免疫效果进行评价。结果显示亚单位菌苗对于猪传染性胸膜肺炎有显著的保护力，能明显减轻临床症状和降低肺部损伤，效果优于商品化的细菌灭活苗。

Study on the Protective Efficiency of the Genetic Subunit Bacterin Against Porcine Contagious Pleuropneumonia

The efficacy of a new genetic subunit bacterin containing recombinant Apx toxins and serovar 7 of Actinobacillus pleuropneumoniae was determined. Weaned piglets were vaccinated twice intramuscularly with the new genetic subunit bacterin or commerical trivalent formalin-inactivated A. pleuropneumoniae. The antibody of each antigen was detected each week after first immunization. Piglets were challenged by percutaneous intratracheal injection with serovar 1. The protective efficacy of immunization was evaluated by clinical, bacteriological, serological and post-mortem examination. The results suggest that immunization with subunit bacterin could reduce clinical sign and lung lesion, which is more efficacious than bacterins.